Advertisement

Radiation and Environmental Biophysics

, Volume 13, Issue 2, pp 79–88 | Cite as

A nondestructive method for measuring electrical conductivity of intracellular matter of tissue in situ

  • J. Krupa
  • J. Terlecki
Article

Summary

A nondestructive method has been developed for measuring electrical conductivity of intracellular matter. The method is based on easily measurable electric parameters of cells in suspension. Experiments were made using suspensions of erythrocytes in a physiological salt solution. Results confirmed suitability of the method for determining conductance of the cytoplasm and were in close agreement with those reported by other authors. The method allows the determination of intracellular conductivity in tissue as well.

Keywords

Electrical Conductivity Salt Solution Environmental Physic Close Agreement Electric Parameter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bielicz, E., Terlecki, J., Fiutak, J., Krupa, J.: Electric admittance and dielectric constant of membrane coated ellipsoidal particle suspension, studia biophysica (in press)Google Scholar
  2. 2.
    Chaplin, H. J., Mollison, P. L., Vetter, H.: Body vencus haematocrit ratio - its constancy over a wide haematocrit range. J. clin. Invest.32, 1309 (1953)Google Scholar
  3. 3.
    Chelidze, T. L., Kidnadze, V. D., Kewlishvili, G. E.: Dielectric spectroscopy of blood. IV. Dielectric of blood after action of physical and chemical agents. Biophysica3, 479–483 (1974)Google Scholar
  4. 4.
    Cole, K. S., Cole, R. H.: Dispersion and absorption in dielectrics. I. Alternating current characteristic. J. chem. Phys.9, 341–351 (1941)Google Scholar
  5. 5.
    Daszyński, J.: Methods of bloods preservation. Clinical transfusiology, pp. 49–70. Warszawa: PZWL 1971Google Scholar
  6. 6.
    Fiutak, J., Terlecki, J.: Relationship between electroconductance of cell suspension and electric properties of the cell. Biophysica5, 873–877 (1973)Google Scholar
  7. 7.
    Krupa, J.: Automatic apparatus for measuring haematocrit and conductance of erythrocytes interior. Part I: Study of measuring method. Digest of the XICMBE, p. 85. Dresden: Dewag 1973Google Scholar
  8. 8.
    Krupa, J., Kalinowski, M.: Über den Einfluß der Konservierungsdauer auf die elektrische Leitfähigkeit des Erythrozytenplasma. Folia haemat (Lpz.)99, 273–278 (1973)Google Scholar
  9. 9.
    Krupa, J., Kwiatkowski, B., Terlecki, J.: Methode zur Leitfähigkeitsbestimmung des Inneren der menschlichen Erythrozyten auf der Grundlage der Messung elektrischer Größen der Suspension. Biophysik8, 227–236 (1972)Google Scholar
  10. 10.
    Krupa, J., Kwiatkowski, B., Terlecki, J.: A contactless method of measuring electrical conductivity inside cells. Post. Fiz. Med.1–2, 129–137 (1973)Google Scholar
  11. 11.
    Novotny, P.: A simple rotary disintegrator for microorganisms and animal tissues. Nature (Lond.)25, 354 (1964)Google Scholar
  12. 12.
    Pauly, W., Schwan, H. P.: Über die Impedanz einer Suspension von kugelförmigen Teilchen mit einer Schale. Z. Naturforsch.14b, 125–131 (1959)Google Scholar
  13. 13.
    Pauly, H.: Über den physikalisch-chemischen Zustand des Wassers und der Elektrolyte in der lebenden Zelle. Biophysik10, 7–32 (1973)Google Scholar
  14. 14.
    Schwan, H. P.: Electrical properties of tissue and cell suspensions. Advanc. biol. med. Phys.5, 147–209 (1957)Google Scholar
  15. 15.
    Silver, G. A., Strauss, J., Misrahy, G. A.: Electrical impedance of isolated amnion. Biophys. J.5, 855–865 (1965)Google Scholar
  16. 16.
    Terlecki, J., Krupa, J.: Experimental verification of the theory of electrical conductivity of cellular suspensions. studia biophysica38, 1–8 (1973)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • J. Krupa
    • 1
    • 2
  • J. Terlecki
    • 1
    • 2
  1. 1.Institute of Automation and RadiocommunicationMarine UniversityGdyniaPoland
  2. 2.Department of Physics and BiophysicsMedical Academy of GdańskPoland

Personalised recommendations