Summary
The influence of hyperthermia on the survival of irradiated yeast cells of different species has been studied. The experiments reported in the paper have shown: (1) simultaneous action of ionizing radiation and high temperatures appeared to increase the radiation response by a factor of approximately 2.7 for diploid and only by a factor of 1.5 for haploid cells of wild-type; (2) the combined action of high temperature and ionizing radiation had no synergistic effect for rad51 mutant diploid yeast cells; (3) heating before or after irradiation did not alter the radiation response of yeast cells; (4) enhancement of yeast cell sensitivity by simultaneous action of hyperthermia and239Pu-α-particles was negligible; (5) the magnitude and the rate of liquid holding recovery is lowered with increasing of irradiation temperature. On this basis, it was concluded that possible mechanism for thermal sensitization of yeast cells may involve the reduced capacity of cells to recover damages resulted from the combined action of both modalities.
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References
Alfieri, A. A., Hahn, E. W., Kim, J. J.: The relationship between the time of fractionated and single doses of radiation and hyperthermia on the sensitization of an in vivo mouse tumor. Cancer36, 893–903 (1975).
Ben-Hur, E.: Mechanisms of the synergistic interaction between hyperthermia and radiation in cultured mammalian cells. J. Radiat. Res. (Tokyo)17, 92–98 (1976)
Ben-Hur, E., Elkind, M. M., Bronk, B. V.: Thermally enhanced radio-response of cultured Chinese hamster cells - Inhibition of repair of sublethal damage and enhancement of lethal damage. Radiat. Res.58, 38–51 (1974)
Crile, G.: The effect of heat and radiation on cancers implanted on feet of mice. Cancer Res.23, 372–380 (1963)
Dietzel, F.: Tumor und Temperatur. München-Berlin-Wien: Urban u. Schwarzenberg 1975
Dietzel, F., Ringleb, D., Schneider, U., Wricke, H.: Microwaves in radiotherapy of tumors - alternative to heavy particles? Strahlentherapie149, 438–441 (1975)
Gerner, E. W., Leith, J. T.: Interaction of hyperthermia with radiations of different linear energy transfer. Int. J. Radiat. Biol.31, 283–288 (1977)
Gerner, E. W., Leith, J. T., Boone, M. L. M.: Mammalian cell survival response following irradiation with 4 MeV X-rays or accelerated helium ions combined with hyperthermia. Radiology119, 715–720 (1976)
Hahn, E. W., Alfieri, A. A., Kim, J. H.: Increased cures using fractionated exposures of X-irradiation and hyperthermia in the local treatment of the Ridgway osteogenic sarcoma in mice. Radiology113, 199–202 (1974)
Hahn, E. W., Candada, T. R., Alferi, A. A., McDonald, J. C.: The radiation enhancement factor (REF) of hyperthermia in combination with fast neutrons on local tumor response. Radiat. Res.59, 141 (1974)
Hofer, K. G., Choppin, D. A., Hofer, M. G.: Effect of hyperthermia on the radiosensitivity of normal and malignant cells in mice. Cancer38, 279–287 (1976)
Kiefer, J., Kraft-Weyrather, W., Hlawica, M. M.: Cellular radiation effects and hyperthermia. Influence of exposure temperatures on survival of diploid yeast irradiated under oxygenated and hypoxic conditions. Int. J. Radiat. Biol.30, 293–300 (1976)
Kiefer, J.: Cellular radiation effects and hyperthermia. Influence of postexposure incubation conditions. Int. J. Radiat. Biol.33, 89–94 (1978)
Kim, S. H., Kim, J. H., Hahn, E. W.: The radiosensitization of hypoxic tumor cells by hyperthermia. Radiology114, 727–728 (1975)
Korogodin, V. I.: The problems of postirradiation recovery (in Russian). Moscow: Atomizdat 1966
Li, G. C., Evans, R. C., Hahn, G. M.: Modification and inhibition of potentially lethal X-ray damage by hyperthermia. Radiat. Res.67, 491–501 (1976)
Nakai, S., Matsumoto, S.: Two types of radiation-sensitive mutants in yeast. Mutat. Res.4, 129–136 (1967)
Patrick, M. H., Haynes, R. H.: Dark recovery phenomena in yeast. II. Conditions that modify the recovery process. Radiat. Res.23, 564–579 (1964)
Petin, V. G.: Comparative studying of yeast cell inactivation and recovery afterα- andγ-irradiation (in Russian). Radiobiologiya9, 421–426 (1969)
Petin, V. G.: RBE of densely ionizing radiations and recovery of cells (in Russian). Medizinskaya radiologiya22, 8–12 (1977)
Petin, V. G.: Sensitivity of yeast cells to a simultaneous action of ionizing radiation and high temperature (in Russian). Radiobiologiya17, 360–366 (1977)
Petin, V. G., Kabakova, N. M.: A comparative study of the RBE ofα-irradiation of yeast cells at different stages of growth (in Russian). Radiobiologiya17, 31–36 (1977)
Petin, V. G., Myasnik, M. N.: Analysis ofα-particle use for inhibition of bacterial cell recovery (in Russian). Radiobiologiya12, 85–91 (1972)
Robinson, J. E., Wizenberg, M. J.: Thermal sensitivity and the effect of elevated temperatures on the radiation sensitivity of Chinese hamster cells. Acta Radiol. (Stockh.)13, 241–248 (1974)
Robinson, J. E., Wizenberg, M. J., McCready, W. A.: Combined hyperthermia and radiation suggest an alternative to heavy particle therapy for reduced oxygen enhancement ratios. Nature251, 521–522 (1974)
Saladino, C., Ben-Hur, E.: Heat-enhanced radioresponse in HeLa cells. Isr. J. Med. Sci.12, 134–138 (1976)
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Petin, V.G., Berdnikova, I.P. Effect of elevated temperatures on the radiation sensitivity of yeast cells of different species. Radiat Environ Biophys 16, 49–61 (1979). https://doi.org/10.1007/BF01326896
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DOI: https://doi.org/10.1007/BF01326896