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Radiation and Environmental Biophysics

, Volume 14, Issue 4, pp 285–294 | Cite as

Radiosensitivity and recovery of mouse L cells during the cell cycle

  • J. Fidorra
  • W. A. Linden
Article

Summary

Mouse fibroblasts, subline L-929 F were synchronized by mitotic detachment. The synchronized cell cultures were irradiated with 200 kVp X-rays at different time after mitosis, and age reponse functions and dose effect curves were determined using the colony test. The cell age in the mitotic cycle was obtained from a computer analysis of flow cytometric DNA histograms. Both intrinsic radiosensitivity 1/D0 and extrapolation numbern were found to vary during the cell cycle. TheD0 has a maximum value of 176 ± 1 rad in the middle ofG1 phase and a minimum of 71 ± 1 rad at theS/G2 transition, while the extrapolation number is rather constant from the beginning ofG1 phase (1.9 ± 0.1) to the middle ofS phase (2.3 ± 0.1) and reaches a steep maximum of 9.3 ± 1.1 atS/G2 transition. The values ofn in the various phases of cell cycle are compared with the respective values of the recovery factorγ determined after fractionated irradiation. - Cell survival after a single dose of 616 rad has minima for irradiation atG1/S transition and in earlyG2 phase; the survival in earlyG2 being about 40 times smaller than in earlyG1 phase. Implications for a cell cycle specific therapy are discussed.

Keywords

Cell Cycle Single Dose Cell Survival Environmental Physic Computer Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • J. Fidorra
    • 1
  • W. A. Linden
    • 1
  1. 1.Institut für Biophysik und StrahlenbiologieUniversität HamburgHamburg 20Germany

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