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Human glioblastoma cells produce 77 amino acid interleukin-8 (IL-877)

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Summary

The production of interleukin 8 (IL-8), a neutrophil chemotactic factor, and its amino acid sequence were examined in glioblastoma cell linesin vitro. Neutrophil chemotactic activity was demonstrated in 9 conditioned media of 15 human glioblastoma cell lines. Tumor necrosis factor (TNF)-α stimulated secretion of the activity in 7 lines and induced secretion in 4 other lines. ELISA quantification disclosed that the conditioned media contained interleukin 8 (IL-8) in an amount equivalent to the chemotactic activity. The IL-8 secretion increased with the stimulation by TNF-α. Northern blot analysis and the RT-PCR method confirmed expression of mRNA in the glioblastoma cells and its augmentation by TNF-α and/or IL-β. Reversed-phase HPLC following ion-exchange chromatography revealed that the chemotactic activity was a single peptide, which was determined to be IL-8 by the retention time and ELISA. Furthermore, amino acid analysis disclosed that a major part of the glioblastoma-cell derived IL-8 peptide was 77 amino acid IL-8 (IL-877; with the N-terminal sequence AVLPRSAKELRCQCI-).

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Authors and Affiliations

  1. Department of Neurosurgery, Hokkaido University School of Medicine, Sapporo, Japan

    Mitsuhiro Tada, Yutaka Sawamura, Shirou Sakuma & Hiroshi Abe

  2. Department of Antibiotics and Humoral Immunity, National Institute of Health, Tokyo, Japan

    Kazuo Suzuki & Yoshio Yamakawa

  3. Neurosurgery Service University Hospital (CHUV), CH-1011, Lausanne, Switzerland

    Erwin van Meir & Nicolas de Tribolet

Authors
  1. Mitsuhiro Tada
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  2. Kazuo Suzuki
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  3. Yoshio Yamakawa
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  4. Yutaka Sawamura
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  5. Shirou Sakuma
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  6. Hiroshi Abe
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  7. Erwin van Meir
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  8. Nicolas de Tribolet
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Tada, M., Suzuki, K., Yamakawa, Y. et al. Human glioblastoma cells produce 77 amino acid interleukin-8 (IL-877). J Neuro-Oncol 16, 25–34 (1993). https://doi.org/10.1007/BF01324831

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