Summary
Thorotrast is a X-ray contrast medium which has been used in the ears 1930–1950. It is hardly ever eliminated from the body and is stored in the organs of the reticulohistiocytic system (RHS). Since Thorotrast contains all radionuclides of the thorium decay chain, the effect of internal irradiation by these radionuclides can be investigated in the so called “Thorotrast patients”. It is the purpose of this study to determine the radiation dose absorbed by the lymphocytes of the peripheral blood of these persons, and to correlate it with their chromosome aberration rate.
Part I of the paper describes the physical aspects of dose evaluation. The chromosome aberration rates of Thorotrast patients were subjected to a repeated statistical test. They show a logarithmic normal distribution at a mean value of about seven dicentric chromosomes per 100 cells. The corresponding dose has to be determined. The method for evaluating size and distribution of Thorotrast conglomerates in the examined tissue is demonstrated, and the function and resolution power of the applied “texture analysing system” (TAS) is described. Size-distributions of Thorotrast conglomerates of lymph nodes from the neck, the lung and from the lower aorta (bifurcatio) are given.
In the lymphatic system more than 90% of the absorbed dose in tissue is due toα-particles. The computation method of calculating the self-absorption ofα-particles in the conglomerates and of calculating the annual doses in the lymph nodes is described. The values lie between 135 and 1200 rad/year. The contribution to irradiation from outside the lymphatic system can be neglected. The method of dose determination is discussed and compared with microdosimetric methods. With these methods, the dose of a lymphocyte caused by one hit of anα-particle lies between 80 and 920 rad. An energy dissipation does not take place and the total energy emitted acts on the lymphocytes thus inducing chromosome aberrations.
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Booz, J.: Microdosimetric spectra and parameters of low LET-radiations. In: Proceedings of the 5th Symposium on Microdosimetry. Verbania Pallanza 22.–26. Sept. 1975. pp. 311–345. EUR 5452 d-e-f. Luxemburg: EURATOM 1975
Brooks, A. L., Mc Clellan, R. O., Peters, R. F., Mead, D. K.: Effect of size and alpha flux of239PuO2 particles on production of chromosome aberrations in liver of Chinese hamster. In: Biological and environmental effects of low-level radiation. Proceedings of a Symposium, Chicago, 3.–7. Nov. 1975, Vol. 2, pp. 131–142. Wien: International Atomic Energy Agency 1976
Buckton, K. E., Langlands, A. O.: The Edinburgh Thorotrast Series - Report of a Cytogenetic Study. In: Proceedings of the Third International Meeting on the Toxicity of Thorotrast. Risø Report No. 294, pp. 114–125. Copenhagen: Danish Atomic Energy Commission 1973
Büchner, F.: Zur Kontrastuntersuchung von Milz und Leber mit Thoriumdioxid. Klin. Wochenschr.11, 1058–1060 (1932)
Edwards, A. A.: Charged particle fluence spectra and their relationship to dose-effect curves for neutrons. In: Proceedings of the 5th Symposium on Microdosimetry. Verbania Pallanza 22.–26. Sept. 1975. pp. 141–152. EUR 5452 d-e-f. Luxemburg: EURATOM 1975
Grillmaier, R., Muth, H., Oberhausen, E.: Eine Anordnung zur Messung kleiner Thoronkonzentrationen in der Luft. Z. Angew. Phys.18, 112–114 (1964)
ICRP lung-model: Deposition and retention models for internal dosimetry of the human respiratory tract. Task Group on Lung Dynamics. Health Phys.12, 173–207 (1966); Health Phys.13, 1251: Errata (1967)
van Kaick, G., Lorenz, H., Kaul, A.: Malignancies in German Thorotrast patients and estimated tissue dose. Health Phys.35, 127–136 (1978a)
van Kaick, G., Kaul, A., Lorenz, D., Muth, H., Wegener, K., Wesch, H.: Late effects and tissue dose in Thorotrast patients. Recent results of German thorotrast study. In: Late biological effects of ionizing radiation. Vol. 1. Vienna: International Atomic Energy Agency. IAEA-SM-224/716, 263–276 (1978b)
Kato, Y.: Alpha-ray dosage near Thorotrast aggregate. Nippon Acta Radiol.26, 1547–1556 (1967)
Kaul, A.: Physikalische und biologische Probleme bei der Thorotrastapplikation. Habilitationsschrift, Berlin (1969)
Kaul, A., Muth, H.: Thorotrast kinetics and radiation dose. Radiat. Environ. Biophys.15, 241–259 (1978)
Kellerer, A. M.: Consideration on the random traversal of convex, bodies and solutions for general cylinders. Radiat. Res.47, 359–376 (1971)
Kemmer, W., Muth, H., Tranekjer, F., Borkenhagen, U.: Chromosome aberration caused by Thorotrast. In: “Proceedings of the Third International Meeting on the Toxicity of Thorotrast”, Risø Report No. 294, pp. 104–113. Copenhagen: Danish Atomic Energy Commission 1973
Kemmer, W.: Investigations to evaluate the long term effects caused by artificial radiation in man (Thorotrast patients) follow-up study: b, Chromosome aberrations caused by thorotrast. In: Programme biology-health protection. EURATOM, Annual Report, 463–465 (1974)
Kemmer, W., Schmutzler, W., Steinsträßer, A.: Radiation dose and chromosome aberrations in radiotherapy patients. In: Mutagen-induced chromosome damage in man. Evans, H. J., Lloyd, D. C. (eds.) pp. 115–119. Edinburgh: Edinburgh University Press 1978
Muth, H., Oberhausen, E., Kunkel, R., Herzfeld, U.: Expected late effects in Thorotrast patients. In: Proceedings of the Third International Meeting on the Toxicity of Thorotrast. Risø Report No. 294, pp. 320–329. Copenhagen: Danish Atomic Energy Commission 1973
Oldenburg, U., Booz, J.: Mass stopping power and pathlength of neutron produced recoils in tissue and tissue equivalent materials. 1. Neutron Energy ≤ 6 MeV. Joint Nuclear Research Centre Ispra Establishment, Italy, Biology Division. EUR 4786e (1972)
Steinsträßer, A.: Die Strahlenbelastung der Lymphozyten bei Thorotrastträgern. Ein Beitrag zur Problematik der „Biologischen Dosimetrie“ nach Inkorporation von Radionukleiden. Dissertation, Math.-Nat. Fakultät der Universität des Saarlandes, Saarbrücken, 1978
UNSCEAR: Report of the United Nations Scientific Committtee on the Effects of Atomic Radiation (Suppl. No. 13). A/7613. United Nations: New York 1969
Wegener, K., Wesch, H., Kampmann, H., Zahnert, R.: Pathological findings in the RES (liver, spleen, lymph nodes, bone marrow) of thorotrast patients (histological and autoradiographic investigations). In: “Proceedings of the Third International Meeting on the Toxicity of Thorotrast”, Risø Report No. 294, pp. 248–258. Copenhagen: Danish Atomic Energy Commission 1973
Wegener, K., Wesch, H.: Pathological changes of lymph nodes in Thorotrast patients (patho-anatomical, autoradiographical and quantitative investigations). Environ. Res.18, 245–255 (1979)
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Dedicated to Professor Dr. Hermann Muth, Homburg (Saar), on the occasion of his 65th birthday
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Steinsträßer, A. Biophysical investigations of the dose-effect relationship in chromosome aberrations of human lymphocytes caused by Thorotrast deposits. Radiat Environ Biophys 19, 1–15 (1981). https://doi.org/10.1007/BF01324260
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DOI: https://doi.org/10.1007/BF01324260