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Studies on the single-shelled rotavirus receptor with a synthetic peptide derived from the cytoplasmic domain of NS28

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Summary

The nonstructural glycoprotein NS28 of rotaviruses plays an important part in the assembly of double-shelled rotaviruses. C-terminal domains of the protein function as a receptor for single-shelled rotavirus particles at the membrane of the rough endoplasmic reticulum. In the present report we describe studies performed with a synthetic peptide corresponding to amino acid (aa) 160 to 169, the most hydrophilic C-terminal epitope of NS28. An antipeptide serum raised against this peptide demonstrated that this epitope was accessible in infected MA104 cells. Moreover, polymeric peptide was demonstrated to aggregate single-shelled rotavirus particles. This aggregation could be almost completely inhibited by preincubation with monomeric peptide. Our results clearly demonstrate that the epitope corresponding to aa 160–169 is able to bind single-shelled rotavirus particles.

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References

  1. Au KS, Mattion NM, Estes MK (1993) A subviral particle binding domain on the rotavirus nonstructural glycoprotein NS28. Virology 194: 665–673

    Google Scholar 

  2. Bergmann CC, Maass D, Poruchynsky MS, Atkinson PH, Bellamy AR (1989) Topology of the non-structural rotavirus receptor glycoprotein NS28 in the rough endoplasmic reticulum. EMBO J 8: 1695–1703

    Google Scholar 

  3. Chan WK, Au KS, Estes MK (1988) Topography of the simian rotavirus nonstructural glycoprotein (NS28) in the endoplasmic reticulum membrane. Virology 164: 435–442

    Google Scholar 

  4. Hansen G, Mehnert F, Streckert HJ, Werchau H (1992) Monoclonal antipeptide antibodies recognize epitopes upon VP4 and VP7 of simian rotavirus SA11 in infected MA104 cells. Arch Virol 122: 281–291

    Google Scholar 

  5. Kabcenell AK, Poruchynsky MS, Bellamy AR, Greenberg HB, Atkinson PH (1988) Two forms of VP7 are involved in assembly of SA11 rotavirus in the endoplasmic reticulum. J Virol 62: 2929–2941

    Google Scholar 

  6. Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105–132

    Google Scholar 

  7. Maass DR, Atkinson PH (1990) Rotavirus proteins VP7, NS28 and VP4 form oligomeric structures. J Virol 64: 2632–2641

    Google Scholar 

  8. Meyer JC, Bergmann CC, Bellamy AR (1989) Interaction of rotavirus cores with the nonstructural glycoprotein NS28. Virology 171: 98–107

    Google Scholar 

  9. Okada Y, Richardson MA, Ikegami N, Nomoto A, Furuichi Y (1984) Nucleotide sequence of human rotavirus genome segment 10, an RNA encoding a glycosylated virus protein. J Virol 51: 856–859

    Google Scholar 

  10. Patton JT (1994) Rotavirus replication. Curr Top Microbiol Immunol 185: 107–127

    Google Scholar 

  11. Streckert HJ, Brüssow H, Werchau H (1988) A synthetic peptide corresponding to the clevage region of VP3 from rotavirus SA11 induces neutralizing antibodies. J Virol 62: 4265–4269

    Google Scholar 

  12. Streckert HJ, Grunert B, Werchau H (1986) Antibodies specific for the carboxy-terminal region of the major surface glycoprotein of simian rotavirus (SA11) and the human rotavirus (Wa). J Cell Biochem 30: 41–49

    Google Scholar 

  13. Streckert HJ, Werchau H (1988) Three amino acids of a heptapeptide-induced antibody are responsible for recognition of SV40 structural proteins. Immunobiology 177: 149–157

    Google Scholar 

  14. Suzuki H, Konno T, Numazaki Y (1993) Electron microscopic evidence for budding process-independent assembly of double shelled rotavirus particles during passage through endoplasmic reticulum membranes. J Gen Virol 74: 2015–2018

    Google Scholar 

  15. Taylor JA, O'Brian JA, Lord VJ, Meyer JC, Bellamy AR (1993) The RER-localized rotavirus intracellular receptor: a truncated purified soluble form is multivalent and binds virus particles. Virology 194: 807–814

    Google Scholar 

  16. Ward CW, Azad AA, Dyall-Smith MC (1985) Structural homologies between RNA segment 10 and 11 from UK bovine, simian SA11 and human WA rotaviruses. Virology 144: 328–336

    Google Scholar 

  17. Ziegner UHM, Frank I, Bernatowicz A, Starr SE, Streckert HJ (1992) Antibody-dependent cellular cytotoxicity (ADCC) is directed against immunodominant epitopes of the envelope proteins of human immunodeficiency virus 1 (HIV-1) Viral Immunol 5: 273–281

    Google Scholar 

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  1. Abteilung für Medizinische Mikrobiologie und Virologie der Ruhr-Universität Bochum, Bochum, Federal Republic of Germany

    M. Olivo & H. -J. Streckert

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  1. M. Olivo
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  2. H. -J. Streckert
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Olivo, M., Streckert, H.J. Studies on the single-shelled rotavirus receptor with a synthetic peptide derived from the cytoplasmic domain of NS28. Archives of Virology 140, 2151–2161 (1995). https://doi.org/10.1007/BF01323237

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  • DOI: https://doi.org/10.1007/BF01323237

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