Skip to main content
SpringerLink
Log in

Immunochemical localization of a willow storage protein with a poplar storage protein antibody

  • Rapid Communication
  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

Antibodies raised from a 32 kDa storage protein of poplar wood are found to bind specifically to protein bodies in willow wood ray cells when the immunogold method is used. A polypeptide of ca. 32 kDa is also obtained from the willow wood which reacts with the poplar storage protein antibodies when the immunoblotting technique is used. The results indicate that willow possesses a storage protein which appears closely related to that of poplar.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

kDa:

kiloDalton

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

References

  • Adler K, Müntz K (1983) Origin and development of protein bodies in cotyledons ofVicia faba. Planta 157: 401–410

    Google Scholar 

  • Cleve B van, Clausen S, Sauter JJ (1988) Immunochemical localization of a storage protein in poplar wood. J. Plant Physiol 133: 371–374

    Google Scholar 

  • Davey JE, van Staden J (1978) Ultrastructural aspects of reserve protein deposition during cotyledonary cell development inLupinus albus. Z Pflanzenphysiol 89: 259–271

    Google Scholar 

  • Greenwood JS, Stinissen HM, Peumans WJ, Chrispeels MJ (1986)Sambucus nigra agglutinin is located in protein bodies in the phloem parenchyma of the bark. Planta 167: 275–278

    Google Scholar 

  • Saigo RH, Peterson DM, Holy J (1983) Development of protein bodies in oat starchy endosperm. Can J Bot 61: 1206–1215

    Google Scholar 

  • Sauter JJ (1981) Seasonal variation of amino acids and amides in the xylem sap ofSalix. Z Pflanzenphysiol 101: 399–411

    Google Scholar 

  • —, Kloth S (1987) Changes in carbohydrates and ultrastructure in xylem ray cells ofPopulus in response to chilling. Protoplasma 137: 45–55

    Google Scholar 

  • —, Wellenkamp S (1988) Protein storing vacuoles in ray cells of willow wood (Salix caprea L.). IAWA Bulletin n.s. 9: 59–65

    Google Scholar 

  • —, Cleve B van, Apel K (1988) Protein bodies in ray cells ofPopulus×canadensis Moench “robusta”. Planta 173: 31–34

    Google Scholar 

  • — —, Wellenkamp S (1989) Ultrastructural and biochemical results on the localization and distribution of storage proteins in a poplar tree and in twigs of other tree species. Holzforschung 43: 1–6

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Botany Institute, University of Kiel, Kiel

    J. J. Sauter & Barbara van Cleve

Authors
  1. J. J. Sauter
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Barbara van Cleve
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sauter, J.J., van Cleve, B. Immunochemical localization of a willow storage protein with a poplar storage protein antibody. Protoplasma 149, 175–177 (1989). https://doi.org/10.1007/BF01322989

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01322989

Keywords

Search

Navigation