Summary
Cytochemical analysis of the endomembrane system of the chlamydomonad flagellate,Gloeomonas kupfferi (Chlorophyta), reveals distinct compartmentalization. Phosphatase localization shows that: IDPase is located throughout all cisternae of the dictyosome and vesicles associated with the contractile vacuole. Other alkaline phosphatases like TPPase, ATPase and ITPase were localized within the trans-face cisternae and vesicles of the contractile vacuole. IMPase was localized at the plasmamembrane and not within the endomembrane system. Acid phosphatases, incl. CMPase, NADPase and β-glycerophosphatase, were localized in vesicles emerging from the central terminus of the trans-face of the dictyosome and in the peripheral vacuolar network. Silver proteinate labeling was noted in the dictyosome, contractile vacuole and on the anterior plasmamembrane. A summary of endomembrane compartmentalization and a putative interpretation of membrane flow and economy are presented.
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Abbreviations
- ER:
-
endoplasmic reticulum
- IDPase:
-
inosine 5′-diphosphatase
- ITPase:
-
inosine 5′-triphosphatase
- ATPase:
-
adenosine 5′-triphosphatase
- TPPase:
-
thiamine pyrophosphatase
- CMPase:
-
cytidine 5′-monophosphatase
- NADPase:
-
β-nicotinamide adenine diphosphatase
- AcPase:
-
acid phosphatase
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Domozych, D.S. The endomembrane system and mechanism of membrane flow in the green alga,Gloeomonas kupfferi (Volvocales, Chlorophyta) II. A cytochemical analysis. Protoplasma 149, 108–119 (1989). https://doi.org/10.1007/BF01322983
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DOI: https://doi.org/10.1007/BF01322983