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Immunofluorescence localization of chalcone synthase in roots ofPisum sativum L. andPhaseolus vulgaris L. and comparable immunochemical analysis of chalcone synthase from pea leaves

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Summary

Chalcone synthase in roots ofPisum sativum andPhaseolus vulgaris was demonstrated enzymatically and immunochemically. In situ localization by indirect immunofluorescence revealed that chalcone synthase is chiefly present in the lateral regions of the calyptra, in the rhizodermis, and the cortex. In the central cylinder the enzyme protein is no longer detectable a short distance behind the meristem. Chalcone synthase was not found in root tips ofZea mays. Two isoforms of chalcone synthase were separated by chromatofocusing of protein extracts from pea leaves. The two forms differed in their subunit molecular masses. The smaller isoform was not detected in roots.

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Abbreviations

CHS:

chalcone synthase

FITC:

fluorescein isothiocyanate

IgG:

immunoglobulin G

DTE:

dithioerythritol

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Authors and Affiliations

  1. Abteilung für Zellbiologie und Ultrastrukturforschung, Institut für Arterioskleroseforschung, Universität Münster, Münster

    M. Rommeswinkel

  2. Institut für Entwicklungs- und Molekularbiologie der Pflanzen, Universität Düsseldorf, Düsseldorf

    B. Karwatzki

  3. Institut für Pharmazeutische Biologie, Universität Bonn, Bonn

    L. Beerhues

  4. Institut für Botanik, Universität Münster, Schlossgarten 3, D-W-4400, Münster, Federal Republic of Germany

    R. Wiermann

Authors
  1. M. Rommeswinkel
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  2. B. Karwatzki
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  3. L. Beerhues
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  4. R. Wiermann
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Rommeswinkel, M., Karwatzki, B., Beerhues, L. et al. Immunofluorescence localization of chalcone synthase in roots ofPisum sativum L. andPhaseolus vulgaris L. and comparable immunochemical analysis of chalcone synthase from pea leaves. Protoplasma 166, 115–121 (1992). https://doi.org/10.1007/BF01322775

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  • DOI: https://doi.org/10.1007/BF01322775

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