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Ultrastructural studies on Maedi-Visna virus

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Summary

Ultrastructural studies of Maedi-Visna virus (MVV) particles isolated from tissue culture fluids of MVV-infected cells as well as cultured cells infected with MVV were performed. MVV particles are bounded by an envelope with projections loosely attached to its surface. Virions contain a core (sometimes two or more) of conical or ovoid shape enclosing an electron-dense nucleoid which is much smaller in diameter than the core and which can only be seen in ultrathin sections. A distinct core shell is to be found in most of the ultrasectioned particles. Cores, liberated from the virions by detergent treatment, exhibited the same shape as their enveloped counterparts. Budding structures with crescents underlying the cell membrane without an intermediate space seem to be bordered on their cell side by an electron-dense thin layer. Particles obviously representing intervening stages of viral maturation showing parts of the crescents at the viral membrane and empty core shells could be found in single cases.

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References

  1. de Boer, G. F.: Zwoegerziekte virus, the causative agent for progressive interstitial pneumonia (maedi) and meningo-leucoencephalitis (visna) in sheep. Res. Vet. Sci.18, 15–25 (1975).

    Google Scholar 

  2. Bruns, M., Straub, O. C., Weiland, F., Feiler, S.: Detection of antibodies against glycoprotein of Maedi-Visna virus released as soluble antigen in cell cultures. Zbl. Vet. Med.B, 26, 461–468 (1979).

    Google Scholar 

  3. Bruns, M., Frenzel, B.: Isolation of a glycoprotein and two structural proteins of Maedi-Visna virus. Virology97, 207–211 (1979).

    Google Scholar 

  4. Chippaux-Hyppolite, C., Taranger, C., Tamalet, J., Pautrat, G., Brahic, M.: Aspects ultrastructuraux du virus Visna en cultures cellulaires. Ann. Inst. Pasteur123, 409–420 (1972).

    Google Scholar 

  5. Coward, J. E., Harter, D. H., Morgan, C.: Electron microscopic observations of Visna virus-infected cell cultures. Virology40, 1030–1038 (1970).

    Google Scholar 

  6. Coward, J. E., Harter, D. H., Hsu, K. C., Morgan, C.: Ferritin-conjugated antibody labeling of Visna virus. Virology50, 925–930 (1972).

    Google Scholar 

  7. Cutlip, R. C., Laird, G. A.: Isolation and characterization of a virus associated with progressive pneumonia (Maedi) of sheep. Amer. J. Vet. Res.37, 1377–1382 (1976).

    Google Scholar 

  8. Dubois-Dalcq, M., Reese, T. S., Narayan, O.: Membrane changes associated with assembly of Visna virus. Virology74, 520–530 (1976).

    Google Scholar 

  9. Dubois-Dalcq, M., Narayan, O., Griffin, D. E.: Cell surface changes associated with mutation of Visna virus in antibody-treated cell cultures. Virology92, 353 to 366 (1979).

    Google Scholar 

  10. Feller, U., Dougherty, R. M., di Stefano, H. S.: Comparativ morphology of avian and murine leukemia viruses. J. Natl. Cancer Inst.47, 1289–1298 (1971).

    Google Scholar 

  11. Fenner, F.: Classification and nomenclature of viruses. Intervirology7, 1–115 (1976).

    Google Scholar 

  12. Frank, H., Schwarz, H., Graf, T., Schäfer, W.: Properties of mouse leukemia viruses XV. Electron microscopic studies on the organization of Friend leukemia virus and other mammalian C-type viruses. Z. Naturforsch.33 c, 124–138 (1978).

    Google Scholar 

  13. Gelderblom, H., Bauer, H., Ogura, H., Wigand, R., Fischer, A. B.: Detection of oncornavirus-like particles in HeLa cells. I. Fine structure and comparative morphological classification. Int. J. Cancer13, 246–253 (1974).

    Google Scholar 

  14. Giebler, P.: Definierung und Auswertung präparativer Ultrazentrifugierungen mit Hilfe des “Performance Index (p.i.)”. Z. Naturforsch.13b, 238–241 (1958).

    Google Scholar 

  15. Gudnadóttir, M., Pálsson, P. A.: Successful transmission of Visna by intrapulmonary inoculation. J. inf. Dis.115, 217–225 (1965).

    Google Scholar 

  16. Gudnadóttir, M., Pálsson, P. A.: Transmission of Maedi by inoculation of a virus grown in tissue culture from Maedi-affected lungs. J. inf. Dis.117, 1–6 (1967).

    Google Scholar 

  17. Haase, A. T., Baringer, J. R.: The structural polypeptides of RNA slow viruses. Virology57, 238–250 (1974).

    Google Scholar 

  18. Harter, D. H., Axel, R., Burny, A., Gulati, S., Schlom, J., Spiegelman, S.: The relationship of Visna, Maedi and RNA tumor viruses as studied by molecular hybridization. Virology52, 287–291 (1973).

    Google Scholar 

  19. de Harven, E.: Remarks on the ultrastructure of type A, B and C virus particles. Adv. Virus Res.19, 221–264 (1974).

    Google Scholar 

  20. Macintyre, E. H., Wintersgill, C. J., Vatter, A. E.: Visna virus infection of sheep and human cellsin vitro — an ultrastructural study. J. Cell Sci.13, 173–191 (1973).

    Google Scholar 

  21. Mehta, P. D., Thormar, H.: Comparative studies of Visna and Maedi viruses as antigens. Infect. Immun.11, 829–834 (1975).

    Google Scholar 

  22. Nermut, M. V., Frank, H., Schäfer, W.: Properties of mouse leukemia viruses. III. Electron microscopic appearance as revealed after conventional preparation techniques as well as freeze-drying and freeze-etching. Virology49, 345–358 (1972).

    Google Scholar 

  23. Pautrat, G., Tamalet, J., Chippaux-Hyppolite, C., Brahic, M.: Etude de la structure du virus Visna en microscopie électronique. C. R. Acad. Sc. (Paris)273, 653–655 (1971).

    Google Scholar 

  24. Pautrat, G., de Micco, P., Tamalet, J.: Observation en microscopie électronique du cycle infectieux du virus Visna sur cellules de plexus choroïdes de mouton à des températures inférieures à 37° C. C. R. Acad. Sc. (Paris)283, 211–214 (1976).

    Google Scholar 

  25. Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212 (1963).

    Google Scholar 

  26. Sarkar, N. H., Manthey, W. J., Sheffield, J. B.: The morphology of murine oncornaviruses following different methods of preparation for electron microscopy. Cancer Res.35, 740–749 (1975).

    Google Scholar 

  27. Schwarz, H., Hunsmann, G., Moennig, V., Schäfer, W.: Properties of mouse leukemia viruses. XI. Immunoelectron microscopic studies on viral structural antigens on the cell surface. Virology69, 169–178 (1976).

    Google Scholar 

  28. Scolnick, E., Rands, E., Aaronson, S. A., Todaro, G. J.: RNA-dependent DNA polymerase activity in five RNA viruses: Divalent cation requirements. Proc. Natl. Acad. Sci. U.S.A.67, 1789–1796 (1970).

    Google Scholar 

  29. Sigurdsson, B., Thormar, H., Pálsson, P. A.: Cultivation of Visna virus in tissue culture. Arch. ges. Virusforsch.10, 368–381 (1960).

    Google Scholar 

  30. Stockem, W.: Die Eignung von Pioloform F für die Herstellung elektronemmikroskopischer Trägerfilme. Mikroskopie26, 185–189 (1970).

    Google Scholar 

  31. Stowring, L., Haase, A. T., Charman, H. P.: Serological definition of the lentivirus group of retroviruses. J. Virol.29, 523–528 (1979).

    Google Scholar 

  32. Straub, O. C.: Die Visna/Maedi-Krankheiten des Schafes. Tierärztl. Umschau25, 373–375 (1970).

    Google Scholar 

  33. Takemoto, K. K., Mattern, C. F. T., Stone, L. B., Coe, J. E., Lavelle, G.: Antigenic and morphological similarities of progressive pneumonia virus, a recently isolated “slow virus” of sheep, to Visna and Maedi viruses. J. Virol.7, 301–308 (1971).

    Google Scholar 

  34. Thormar, H.: An electron microscope study of tissue cultures infected with Visna virus. Virology14, 463–475 (1961).

    Google Scholar 

  35. Thormar, H., Cruickshank, J. G.: The structure of Visna virus studied by the negative staining technique. Virology25, 145–148 (1965).

    Google Scholar 

  36. Weiland, F., Behrens, H.: Zum Auftreten der Progressiven Interstitiellen Pneumonie (Maedi) des Schafes in Norddeutschland. Dtsch. Tierärztl. Wschr.77, 373–376 (1970).

    Google Scholar 

  37. Weiss, M. J., Zeelon, E. P., Sweet, R. W., Harter, D. H., Spiegelman, S.: Immunological cross-reactions of the major internal protein component from “slow” viruses of sheep. Virology76, 851–854 (1977).

    Google Scholar 

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  1. M. Bruns

    Present address: Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Hamburg, Germany

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  1. Federal Research Institute for Animal Virus Diseases, Tübingen, Germany

    F. Weiland & M. Bruns

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  1. F. Weiland
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Weiland, F., Bruns, M. Ultrastructural studies on Maedi-Visna virus. Archives of Virology 64, 277–285 (1980). https://doi.org/10.1007/BF01322707

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