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Molecular analysis of rice ragged stunt oryzavirus segment 9 and sequence conservation among isolates from Thailand and India

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Summary

Nucleotide sequences of rice ragged stunt virus (RRSV) genome segment 9 (S9) from a Thai and an Indian isolate were determined. Both sequences are 1132 bp long, contain a single large open reading frame (ORF) spanning nucleotide residues 14 to 1027 and are capable of encoding a protein of 38.5K. The two isolates are 94.6% and 99.4% identical at the nucleotide and amino acid level, respectively. The authenticity of these coding sequences was confirmed by identifying a ∼38K protein in the RRSV particle with an N-terminal amino acid sequence identical to that inferred from the S9 ORF. Furthermore, cDNA of S9 from each isolate incorporated into the bacterial expression vector pGEX3-X produced a fusion protein that reacted with antibodies raised against purified RRSV particles. Cleaving these fusion proteins with protease factor X liberated a ∼38K polypeptide.

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Authors and Affiliations

  1. CSIRO Division of Plant Industry, Canberra, Australia

    N. M. Upadhyaya, M. Yang & P. M. Waterhouse

  2. Department of Plant Pathology, Kasetsart University, Nakhon Pathom, Thailand

    W. Kositratana

  3. Directorate of Rice Research Hyderabad, Andhra Pradesh, India

    A. Ghosh

Authors
  1. N. M. Upadhyaya
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  2. M. Yang
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  3. W. Kositratana
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  4. A. Ghosh
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  5. P. M. Waterhouse
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Additional information

The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession numbers L38899 (for RRSVS9-T) and L38900 (for RRSVS9-I).

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Upadhyaya, N.M., Yang, M., Kositratana, W. et al. Molecular analysis of rice ragged stunt oryzavirus segment 9 and sequence conservation among isolates from Thailand and India. Archives of Virology 140, 1945–1956 (1995). https://doi.org/10.1007/BF01322684

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  • DOI: https://doi.org/10.1007/BF01322684

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