Summary
Caffeine is a potent inhibitor of cell plate formation in dividing plant cells. Previous studies living cells reveal that the drug always permits the cell plate to arise and grow normally until about 80% complete, but then causes it to break down. In the present investigation we examine this formation/degradation cycle at the ultrastructure level. Our results show that during the formation phase the caffeine treated plate is indistinguishable from untreated controls. Phragmoplast microtubules arise and align in the interzone, Golgi vesicles are produced and aggregate in a line that defines the young cell plate, and considerable fusion of these vesicles occurs to form islands of plate material. However, under the influence of caffeine these islands do not fuse to form the enlarged lamellar expanses characteristic of maturing cell plates. Instead, the partially fused material reverts to small vesicles which appear to become resorbed by the cellular membrane systems. The resorption process continues leaving no evidence of the previously developing plate, although occasionally we observe a stub of fused vesicles attached to the parent wall. Following cell plate disintegration the reformed nuclei move close together and occupy the central region of the cell. These observations focus attention on the consolidation phase of cell plate formation as the one being maximally affected by caffeine.
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References
Amrhein N (1974) Evidence against the occurrence of adenosine 3′5′cAMP in higher plants. Planta 118: 241–258
Augustine GL, Charlton MP, Smith SJ (1987) Calcium action in synaptic transmitter release. Annu Rev Neurosci 10: 633–693
Becerra J, Lopez-Saez JF (1978) Effects of caffeine, calcium and magnesium on plant cytokinesis. Exp Cell Res 111: 310–308
Bianchi CP (1968) Pharmacological action on excitation-contraction coupling in striated muscle. Fed Proc 27: 126–131
Bonsignore CL, Hepler PK (1985) Caffeine inhibition of cytokinesis: dynamics of cell plate formation-deformation in vivo. Protoplasma 129: 28–35
Butcher RW, Sutherland EW (1962) Adenosine-3′5′-phosphate in biological materials. 1. Purification and properties of 3′5′-nucleotide phosphodiesterase and use of this enzyme to characterize adenosine 3′5 phosphate in human urine. J Biol Chem 237: 1244–1250
Callewaert G, Gleeman L, Morad M (1989) Caffeine-induced Ca2+ extrusion via Na+-Ca2+ exchanger in cardiac myocytes. Am J Physiol 257: C147-C152
Chen TL, Wolniak SM (1987) Lithium induces cell plate dispersion during cytokinesis inTradescantia. Protoplasma 141: 56–63
Clayton L, Lloyd CW (1985) Actin organization during the cell cycle in meristematic plant cells. Exp Cell Res 156: 231–238
De Lisle RC, Williams JA (1986) Regulation of membrane fusion in secretory exocytosis. Annu Rev Physiol 48: 225–238
Domozych DS (1989) The disruption of dictyosomal integrity by caffeine in the green alga flagellate,Gloeomonas kupfferi. J Cell Sci 93: 375–383
Endo M (1977) Calcium release from sarcoplasmic reticulum. Physiol Rev 57: 71–108
Fain J, Malbon C (1979) Regulation of adenylate cyclase by adenosine. Mol Cell Biochem 25: 143–169
Gonzalez-Fernandez A, Lopez-Saez JF (1980) The effect of adenosine on caffeine inhibition of plant cytokinesis. Environ Exp Bot 20: 455–461
Gunning BES (1982) The cytokinetic apparatus: its development and spatial regulation. In: Lloyd CW (ed) The cytoskeleton in plant growth and development. Academic Press, New York, pp 230–288
—, Wick SM (1985) Prepophase bands, phragmoplasts and spatial control of cytokinesis. J Cell Sci [Suppl] 2: 157–179
Hardham AR, McCully ME (1982) Reprogramming of cells following wounding in pea (Pisum sativum L.) roots. I. Cell division and differentiation of new vascular elements. Protoplasma 112: 143–151
Hatano S, Oosawa F (1971) Movement of cytoplasm in plasmodial fragments obtained by caffeine treatment. I. Its Ca++ sensitivity. J Physiol Soc Jpn 33: 589–591
Hepler PK (1982) Endoplasmic reticulum in the formation of the cell plate and plasmodesmata. Protoplasma 111: 121–133
— (1985) Calcium restriction prolongs metaphase in dividingTradescantia stamen hair cells. J. Cell Biol 100: 1363–1368
—, Newcomb EH (1967) Fine structure of cell plate formation in the apical meristem ofPhaseolus roots. J Ultrastruct Res 19: 498–513
— Palevitz BA, Lancelle SA, McCauley MM, Lichtscheidl I (1990) Cortical endoplasmic reticulum in plants: a review. J Cell Sci (in press)
Jones MGK, Payne HL (1978) Cytokinesis inImpatiens balsamina and the effect of caffeine. Cytobios 20: 79–91
Kakimoto T, Shibaoka H (1988) Cytoskeletal ultrastructure of phragmoplast-nuclei complexes isolated from cultured tobacco cells. Protoplasma [Suppl] 2: 95–103
Lancelle SA, Hepler PK (1986) Cutinase pretreatment greatly enhances osmium tetroxide-potassium ferricyanide staining of stamen hair cells. Can J Bot 64: 1509–1512
Lopez-Saez JF, Mingo R, Gonzalez-Fernandez A (1982) ATP level and caffeine efficiency on cytokinesis inhibition in plants. Eur J Cell Biol 27: 185–190
—, Risueno MC, Gimenez-Martin G (1966) Inhibition of cytokonesis in plant cells. J Ultrastruct Res 14: 85–94
Palevitz BA (1987) Accumulation of F-actin during cytokinesis inAllium. Correlation with microtubule distribution and the effects of drugs. Protoplasma 141: 24–32
Pareyre C, Lasselain MJ, Deysson G (1979) The mechanism of cytokinesis in plant cells: action of caffeine on the kinetics of a root meristem cell population. Cytobios 26: 153–166
Paul DC, Goff CW (1973) Comparative effects of caffeine, its analogues and calcium deficiency on cytokinesis. Exp Cell Res 78: 399–413
Pickett-Heaps JD (1969) Preprophase microtubule bands in some abnormal mitotic cells of wheat. J Cell Sci 4: 397–420
Röper W, Röper S (1977) Centripetal wall formation in roots ofVicia faba after caffeine treatment. Protoplasma 93: 89–100
Saunders MJ, Hepler PK (1981) Localization of membrane-associated calcium following cytokinin treatment inFunaria using chlorotetracycline. Planta 152: 272–281
Sawamura S (1952) The effect of caffeine and 8-oxyquinoline on the mitosis ofTradescantia cell in vivo. Bull Utsunomiya Univ 2: 167–175
Sekar MC, Hokin LE (1986) The role of phosphoinositides in signal transduction. J Memb Biol 89: 193–210
Speksnijder JE, Miller AL, Weisenseel MH, Chen TH, Jaffe LF (1989) Calcium buffer injections block fucoid egg development by facilitating calcium diffusion. Proc Natl Acad Sci USA 86: 6607–6611
Steer MW, Steer JL (1989) Pollen tube tip growth. Tansley Review no 16. New Phytol 111: 323–358
Stossel TP (1989) From signal to pseudopod. How cells control cytoplasmic actin assembly. J Biol Chem 264z: 18261–18264
Weber A, Herz R (1968) The relationship between caffeine contraction of intact muscle and the effect of caffeine on reticulum. J Gen Physiol 52: 750–759
Wolniak SM, Bart KM (1985) The buffering of calcium with quin2 reversibly forestalls anaphase onset in stamen hair cells ofTradescantia. Eur J Cell Biol 39: 33–40
—, Hepler PK, Jackson WT (1980) Detection of membrane-calcium distribution during mitosis inHaemanthus endosperm with chlorotetracyline. J Cell Biol 87: 23–32
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Dedicated to the memory of Professor Oswald Kiermayer
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Hepler, P.K., Bonsignore, C.L. Caffeine inhibition of cytokinesis: Ultrastructure of cell plate formation/degradation. Protoplasma 157, 182–192 (1990). https://doi.org/10.1007/BF01322651
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DOI: https://doi.org/10.1007/BF01322651