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Effects of estrogen replacement on insulin-like growth factor I concentrations in serum and bone tissue and on interleukin 1 secretion from spleen macrophages in oophorectomized rats

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Summary

Oophorectomy (OOX) has been known to increase bone turnover, but its precise mechanism is not fully understood. In order to further investigate the mechanism, we determined insulinlike growth factor I (IGF-I) concentrations in serum and bone tissue and interleukin 1 (IL-1) release from spleen macrophages in oophorectomized rats because it has been demonstrated that IGF-I stimulates bone formation and IL-1 stimulates bone resorption.

Female 8-week-old Wistar rats were divided into four groups: (1) control, (2) OOX, (3) OOX given estradiol, and (4) control given estradiol. Ten μg/kg of 17β-estradiol was given daily by subcutaneous injection. After 5 weeks of treatment, IGF-I concentrations in the extract from right femur and in serum were determined by specific radioimmunoassay. IL-1 activity released from lipopolysaccharide (LPS)-stimulated spleen macrophages was determined by bioassay. IGF-I contents in the femur and IGF-I concentrations in serum in oophorectomized rats were significantly higher than those in control rats. Treatment by estradiol inhibited the increase in IGF-I concentrations both in femur and in serum. IL-1 release from LPS-stimulated spleen macrophages in oophorectomized rats was increased, and treatment by estradiol also inhibited the stimulated IL-1 release. The ash weights and the calcium contents of left femur in oophorectomized rats were lower than those in control rats. These results suggest that both IGF-I and IL-1 may be involved in the mechanism of the regulation of bone turnover in oophorectomized rats.

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Abbreviations

IGF:

insulinlike growth factor

IL:

interleukin

OOX:

oophorectomy

LPS:

lipopolysaccharide

PTH:

parathyroid hormone

BGP:

bone gla protein

MEM:

minimum essential medium

FCS:

fetal calf serum

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Authors and Affiliations

  1. Department of Geriatrics, Faculty of Medicine, University of Tokyo, 113, Tokyo, Japan

    Fumiyasu Sato, Yasuyoshi Ouchi, Akihiro Masuyama, Tetsuro Nakamura, Takayuki Hosoi, Yasushi Okamoto & Hajime Orimo

  2. Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, 113, Tokyo, Japan

    Norio Sasaki

  3. Department of Laboratory Medicine, Tokyo Metropolitan Geriatric Hospital, 173, Tokyo, Japan

    Masataka Shiraki

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  1. Fumiyasu Sato
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  2. Yasuyoshi Ouchi
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  3. Akihiro Masuyama
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  4. Tetsuro Nakamura
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  5. Takayuki Hosoi
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  6. Yasushi Okamoto
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  7. Norio Sasaki
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  9. Hajime Orimo
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Sato, F., Ouchi, Y., Masuyama, A. et al. Effects of estrogen replacement on insulin-like growth factor I concentrations in serum and bone tissue and on interleukin 1 secretion from spleen macrophages in oophorectomized rats. Calcif Tissue Int 53, 111–116 (1993). https://doi.org/10.1007/BF01321888

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  • DOI: https://doi.org/10.1007/BF01321888

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