Advertisement

Archives of Virology

, Volume 122, Issue 1–2, pp 201–206 | Cite as

Adaptation of transmissible gastroenteritis virus to growth in non-permissive Vero cells

  • H. Ishii
  • I. Watanabe
  • M. Mukamoto
  • Y. Kobayashi
  • Y. Kodama
Brief Report

Summary

The CPK cells derived from swine kidney were infected with the attenuated TO-163 strain of transmissible gastroenteritis (TGE) virus, and fused with uninfected Vero cells in the presence of polyethylene glycol. Repeated cocultivation of the fused cells with uninfected Vero cells rendered the virus to grow in Vero cells. The Vero cell-adapted virus acquired the ability to infect and produce cytopathic effects in several other non-permissive cell lines of non-porcine origin. No major differences in viral polypeptides were shown between the Vero cell-adapted TO-163 strain and its parent strain by indirect immunofluorescence and Western blotting using monoclonal and polyclonal antibodies to TGE virus.

Keywords

Polyethylene Glycol Polypeptide Polyclonal Antibody Parent Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Choppin PW, Scheid A (1980) The role of viral glycoproteins in adsorption, penetration, and pathogenicity of viruses. Rev Infect Dis 2: 40–61Google Scholar
  2. 2.
    Frana MK, Behnke JN, Sturman LS, Holmes KV (1985) Proteolytic cleavage of the E 2 glycoprotein of murine coronavirus: host-dependent differences in proteolytic cleavage and cell fusion. J Virol 56: 912–920Google Scholar
  3. 3.
    Furuuchi S, Shimizu Y, Kumagai T (1978) Multiplication of low and high cell culture passaged strains of transmissible gastroenteritis virus in organs of newborn piglets. Vet Microbiol 3: 169- 178Google Scholar
  4. 4.
    Garwes DJ, Pocock DH (1975) The polypeptide structure of transmissible gastroenteritis virus. J Gen Virol 29: 25–34Google Scholar
  5. 5.
    Harada K, Furuuchi S, Kumagai T, Sasahara J (1969) Pathogenecity, immunogenecity and distribution of transmissible gastroenteritis virus in pigs. Natl Inst Anim Health Q 9: 185–192Google Scholar
  6. 6.
    Horzinek MC, Lutz H, Pedersen NC (1982) Antigenic relationships among homologous structural polypeptides of porcine, feline and canine coronaviruses. Infect Immun 37: 1148–1155Google Scholar
  7. 7.
    Ishii H, Yoshikawa Y, Yamanouchi K (1986) Adaptation of the lapinized rinderpest virus to in vitro growth and attenuation of its virulence in rabbits. J Gen Virol 67: 275–280Google Scholar
  8. 8.
    Kemeny LJ, Wiltsey VL, Riley JL (1975) Upper respiratory infection of lactating sow with transmissible gastroenteritis virus following contact exposure to infected piglets. Cornell Vet 65: 352–362Google Scholar
  9. 9.
    Klenk HD, Rott R (1980) Cotranslational and posttranslational processing of viral glycoproteins. Curr Top Microbiol Immunol 90: 19–48Google Scholar
  10. 10.
    Komaniwa H, Fukusho A, Shimizu Y (1981) Micromethod for performing titration and neutralization test of hog cholera virus using established porcine kidney cell strain. Natl Inst Anim Health Q 21: 153–158Google Scholar
  11. 11.
    Pensaert MB, Haelterman EO, Burnstein T (1970) Transmissible gastroenteritis of swine: Virus-intestinal cell interactions. I. Immunofluorescence, histopathology and virus production in the small intestine through the course of infection. Arch Ges Virusforsch 31: 321–334Google Scholar
  12. 12.
    Reynolds DJ, Garwes DJ, Lucey S (1980) Differenciation of canine coronavirus and porcine transmissible gastroenteritisvirus by neutralization with canine, porcine and feline sera. Vet Microbiol 5: 283–290Google Scholar
  13. 13.
    Rohde E, Pauli G, Henning J, Friis RR (1978) Polyethylene glycol-mediated infection with avian sarcoma viruses. Arch Virol 58: 55–59Google Scholar
  14. 14.
    Sturman LS, Ricard CS, Holmes KV (1985) Proteolytic cleavage of the E 2 glycoprotein of murine coronavirus: activation of cell-fusing activity of virions by trypsin and separation of two different 90 K cleavage fragments. J Virol 56: 904–911Google Scholar
  15. 15.
    Underdahl NR, Mebus EL, Stair EL, Rhodes MB, McGill LD, Twiehaus MJ (1974) Isolation of transmissible gastroenteritis virus from lungs of market-weight swine. Am J Vet Res 35: 1209–1216Google Scholar
  16. 16.
    Wege H, Siddell S, Ter Meulen V (1982) The biology and pathogenesis of coronaviruses. Curr Top Microbiol Immunol 99: 165–200Google Scholar
  17. 17.
    Woods RD, Cheville NF, Gallagher JE (1981) Lesions in the small intestine of newborn pigs inoculated with porcine, feline, and canine coronaviruses. Am J Vet Res 42: 1163–1169Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • H. Ishii
    • 1
  • I. Watanabe
    • 1
  • M. Mukamoto
    • 1
  • Y. Kobayashi
    • 1
  • Y. Kodama
    • 1
  1. 1.Ghen CorporationGifu LaboratoryGifu-CityJapan

Personalised recommendations