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Cell fusion caused by herpes simplex virus-1 (HSV-1) strains tsB5 and MP is inhibited at pH 6.7 and pH 7.0

Archives of Virology volume 122, pages 119–131 (1992)Cite this article

Summary

We investigated the effect of different pH conditions on Vero cell cultures infected with herpes simplex virus-1 (HSV-1) wild-type strain KOS, and syncytial mutants HSV-1 HFEM (tsB5) and HSV-1 mp (MP). Cell fusion was inhibited when infected cells were continuously incubated with culture media adjusted to pH 6.7 or pH 7.0. Inhibition of cell fusion was rapidly reversible when infected cell cultures were returned to pH 7.5. The rate of synthesis and cell-surface expression of virus-specified glycoproteins gB, gC, gD, and gH were not affected during continuous incubation at pH 7.0, but they were reduced at pH 6.7 in comparison to pH 7.5. At later hours p.i. however, these glycoproteins continued to accumulate at all tested pH levels. Accumulation of infectious virions was substantially reduced for MP, KOS, andtsB5 at pH 6.7. At pH 7.0, KOS andtsB5 titers were greatly reduced but MP titers were not affected.

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Authors and Affiliations

  1. Department of Veterinary Microbiology and Parasitology, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana, USA

    A. Baghian, M. A. Dietrich & K. G. Kousoulas

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  1. A. Baghian
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  2. M. A. Dietrich
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  3. K. G. Kousoulas
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Baghian, A., Dietrich, M.A. & Kousoulas, K.G. Cell fusion caused by herpes simplex virus-1 (HSV-1) strains tsB5 and MP is inhibited at pH 6.7 and pH 7.0. Archives of Virology 122, 119–131 (1992). https://doi.org/10.1007/BF01321122

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  • DOI: https://doi.org/10.1007/BF01321122

Keywords

  • Cell Culture
  • Infectious Disease
  • Infected Cell
  • Herpes Simplex
  • Vero Cell

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