Summary
Scrapie infectivity is reduced 5–6 logs following filtration through 100,000 MW cut-off filter plus overnight treatment with 6 M urea. These steps, applied to purified human Growth Hormone (hGH), increase the margin of safety of hGH.
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References
Bernoulli C, Siegfried J, Baumgartner G, Regli F, Rabinowicz T, Gajdusek DC, Gibbs CJ Jr (1977) Danger of accidental person-to-person transmission of Creutzfeldt-Jakob disease by surgery. Lancet i: 478–479
Brown P (1985) Virus sterility for human growth hormone. Lancet ii: 729–730
Brown P, Gajdusek DC, Gibbs CJ Jr, Asher DM (1985) Potential epidemic of Creutzfeldt-Jakob disease from human growth hormone therapy. N Engl J Med 313: 728–731
Brown P, Rohwer RG, Gajdusek DC (1984) Sodium hydroxide decontamination of Creutzfeldt-Jakob disease virus. N Engl J Med 310: 727
Brown P, Rohwer RG, Gajdusek DC (1986) Newer data on the investigation of scrapie virus or Creutzfeldt-Jakob disease virus in brain tissue. J Infect Dis 153: 1145–1148
Dickinson AG, Meikle MH, Fraser H (1969) Genetical Control of the concentration of ME7 scrapie agent in the brain of mice. J Comp Pathol 79: 15–22
Duffy P, Wolf J, Collins G, DeVoe AG, Streeten B, Cowen D (1974) Possible person-to-person transmission of Creutzfeldt-Jakob disease. N Engl J Med 290: 692–693
Gibbs CJ Jr, Gajdusek DC, Morris JA (1965) Viral characteristic of the scrapie agent in mice. In: Gajdusek DC, Gibbs CJ Jr, Alpers M (eds) Slow, latent, and temperate virus infections. NINDB2 monograph no. 2. US Government Printing Office, Washington, DC, pp 195–202
Hadlow WJ, Eklund CM, Kennedy RC, Jackson TA, Whitford HW, Boyle CC (1974) Course of experimental scrapie virus infection in the goat. J Infect Dis 129: 559–567
Hunter GD, Gibbons RA, Kimberlin RH, Millson GC (1969) Further studies of the infectivity and stability of extracts and homogenates derived from scrapie affected mouse brains. J Comp Pathol 79: 101–108
Kimberlin RH, Walker CA (1977) Characteristics of a short incubation model of scrapie in the golden hamster. J Gen Virol 34: 295–304
Kimberlin RH, Walker CA, Millson GC, Taylor DM, Robertson PA, Tomlinson AH, Dickinson AG (1983) Disinfection studies with two strains of mouse-passaged scrapie agent. Guidelines for Creutzfeldt-Jakob and related agents. J Neurol Sci 59: 355–369
Lumley-Jones R, Benker G, Salacinski PR, Lloyd TJ, Lowry PJ (1979) Large scale preparation of highly purified pyrogen-free human growth hormone for clinical use. J Endocrinol 82: 77–86
Marsh RF, Castle BE, Dees C, Wide WF (1984) Equilibrium density gradient centrifugation of the scrapie agent in Nycodenz. J Gen Virol 65: 1963–1968
Mould DL, unpublished data. Cited in [10] (
Prusiner SB (1984) Prions: novel infectious pathogens. In: Lauffer MA, Maramorosch K (eds) Advances in virus research. Academic Press, New York, pp 1–56
Reed LJ, Muench H (1938) A simple method of estimating fifty per cent endpoints. Am J Hyg 27: 493–497
Stamp JT, Brotherston JG, Zlotnik I, MacKay JMK, Smith W (1959) Further studies on scrapie. Comp Pathol 69: 268–280
Taylor DM, Dickinson AG, Fraser H, Robertson PA, Salacinski PR, Lowry PJ (1985) Preparation of growth hormone free from contamination with unconventional slow viruses. Lancet ii: 260–262
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Pocchiari, M., Macchi, G., Peano, S. et al. Can potential hazard of Creutzfeldt-Jakob disease infectivity be reduced in the production of human Growth Hormone?. Archives of Virology 98, 131–135 (1988). https://doi.org/10.1007/BF01321014
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DOI: https://doi.org/10.1007/BF01321014