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Integration of cellular-SV 40 DNA and the thermal stability of the recombinant sites

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Summary

We calculated the predicted melting profiles of evolutionary variants of SV 40 and compared the nucleotide sequences of largeT antigen binding sites and recombinant sites for homology. Neither is critically important in selecting a recombinant site for SV 40-SV 40 and SV 40-cellular DNA fragments.

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References

  1. Crawford LV (1980) The T-antigens of simian virus 40 and polyoma virus: their role in transformation. TIBS 5: 39–42

    Google Scholar 

  2. Davoli D, Genoem D, Naussabum AL, Fareed G, Howley PM, Khoury G, Martin MA (1977) Genome structure of reiteration mutants of simian virus 40. Virology 77: 110–124

    Google Scholar 

  3. Fantuzzi LF, Scheidtmann KH, Cesare V (1986) Biochemical properties of a transforming nonkaryotic T-antigen SV 40. Virology 153: 87–95

    Google Scholar 

  4. Fixman M, Freire JJ (1977) Theory of DNA melting curves. Biopolymers 16: 2693–2704

    Google Scholar 

  5. Gotoh O, Tagashira Y (1981) Locations of frequently opening regions on natural DNA and their relation to functional loci. Biopolymers 20: 1043–1058

    Google Scholar 

  6. Gutai MS (1981) Recombination in SV 40-infected cells. Virology 109: 344–352

    Google Scholar 

  7. Gutai MW, Nathans D (1978) Evolutionary variants of simian virus 40. J Mol Biol 126: 275–288

    Google Scholar 

  8. Lee TNH, Brockman WW, Nathans D (1975) Evolutionary variants of simian virus 40: cloned substituted variants containing multiple initiation sites for DNA replication. Virology 66: 53–69

    Google Scholar 

  9. Mayers RM, Rio MD, Robbins AK, Tjian R (1981) SV 40 gene expression is modulated by the cooperative binding of T-antigen to DNA. Cell 25: 373–384

    Google Scholar 

  10. Rio DC, Tjian R (1983) SV 40 T-antigen binding site mutations that affect autoregulation. Cell 32: 1227–1240

    Google Scholar 

  11. Rosenberg M, Segal S, Kuff EL, Singer MF (1977) The nucleotide sequence of repetitive monkey DNA found in defective simian virus 40. Cell 11: 845–857

    Google Scholar 

  12. Stringer JR (1985) Recombination between poly [d(GT).(CA)] sequence in simian virus 40-infected cultured cells. Mol Cell Biol 5: 1247–1259

    Google Scholar 

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Supported in part by Grant CA-11430 from the National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A.

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Rashid, K., White, R.A. Integration of cellular-SV 40 DNA and the thermal stability of the recombinant sites. Archives of Virology 98, 87–90 (1988). https://doi.org/10.1007/BF01321008

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  • DOI: https://doi.org/10.1007/BF01321008

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