Summary
We calculated the predicted melting profiles of evolutionary variants of SV 40 and compared the nucleotide sequences of largeT antigen binding sites and recombinant sites for homology. Neither is critically important in selecting a recombinant site for SV 40-SV 40 and SV 40-cellular DNA fragments.
References
Crawford LV (1980) The T-antigens of simian virus 40 and polyoma virus: their role in transformation. TIBS 5: 39–42
Davoli D, Genoem D, Naussabum AL, Fareed G, Howley PM, Khoury G, Martin MA (1977) Genome structure of reiteration mutants of simian virus 40. Virology 77: 110–124
Fantuzzi LF, Scheidtmann KH, Cesare V (1986) Biochemical properties of a transforming nonkaryotic T-antigen SV 40. Virology 153: 87–95
Fixman M, Freire JJ (1977) Theory of DNA melting curves. Biopolymers 16: 2693–2704
Gotoh O, Tagashira Y (1981) Locations of frequently opening regions on natural DNA and their relation to functional loci. Biopolymers 20: 1043–1058
Gutai MS (1981) Recombination in SV 40-infected cells. Virology 109: 344–352
Gutai MW, Nathans D (1978) Evolutionary variants of simian virus 40. J Mol Biol 126: 275–288
Lee TNH, Brockman WW, Nathans D (1975) Evolutionary variants of simian virus 40: cloned substituted variants containing multiple initiation sites for DNA replication. Virology 66: 53–69
Mayers RM, Rio MD, Robbins AK, Tjian R (1981) SV 40 gene expression is modulated by the cooperative binding of T-antigen to DNA. Cell 25: 373–384
Rio DC, Tjian R (1983) SV 40 T-antigen binding site mutations that affect autoregulation. Cell 32: 1227–1240
Rosenberg M, Segal S, Kuff EL, Singer MF (1977) The nucleotide sequence of repetitive monkey DNA found in defective simian virus 40. Cell 11: 845–857
Stringer JR (1985) Recombination between poly [d(GT).(CA)] sequence in simian virus 40-infected cultured cells. Mol Cell Biol 5: 1247–1259
Author information
Authors and Affiliations
Additional information
Supported in part by Grant CA-11430 from the National Cancer Institute, National Institutes of Health, Bethesda, MD, U.S.A.
Rights and permissions
About this article
Cite this article
Rashid, K., White, R.A. Integration of cellular-SV 40 DNA and the thermal stability of the recombinant sites. Archives of Virology 98, 87–90 (1988). https://doi.org/10.1007/BF01321008
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01321008