Summary
Incorporation of14C-thymidine by cells infected with African swine fever virus (ASFV) occurs in the nucleus. Part of this DNA is transferred to the cytoplasm and becomes resistant to DNAse. The nuclear fraction washed with Triton X 100 retained all labeled DNA and was able to synthesize viral and cellular DNA underin vitro conditions in the presence of the four deoxynucleoside triphosphates, Mg+2, and sucrose. Under similar conditions nuclei from uninfected cells synthesized very little DNA.
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Tabares, E., Botija, C.S. Synthesis of DNA in cells infected with African swine fever virus. Archives of Virology 61, 49–59 (1979). https://doi.org/10.1007/BF01320591
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DOI: https://doi.org/10.1007/BF01320591