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Isolation and cell culture propagation of rotaviruses from turkeys and chickens

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Summary

Rotaviruses were detected by electron microscopy in the faeces of turkey poults and broiler chickens with diarrhoea. Apparently symptomless infection was also observed in broilers. The avian rotaviruses could not be isolated in cell cultures by conventional techniques, but were adapted to serial growth in chick cell cultures following trypsin treatment of the virus and the cells. Immunofluorescence studies showed that the avian and mammalian rotaviruses are antigenically related. Antibodies to rotavirus were widespread in sera collected from turkeys, chickens and ducks.

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References

  1. Almeida, J. D., Hall, T., Banatvala, J. E., Totterdell, B. M., Chrystie, I. L.: The effect of trypsin on the growth of rotavirus. J. gen. Virol.40, 213–218 (1978).

    Google Scholar 

  2. Babiuk, L. A., Mohammed, K., Spence, L., Fauvel, M., Petro, R.: Rotavirus isolation and cultivation in the presence of trypsin. J. clin. Microbiol.6, 610–617 (1977).

    Google Scholar 

  3. Bergeland, M. E., McAdaragh, J. P., Stotz, I.: Rotaviral enteritis in turkey poults. Proc. 26th Western Poultry Disease Conference, University of California, Davis, 129–130 (1977).

    Google Scholar 

  4. Bryden, A. S., Davies, H. A., Thouless, M. E., Flewett, T. H.: Diagnosis of rotavirus infection by cell culture. J. med. Microbiol.10, 121–125 (1977).

    Google Scholar 

  5. Chrystie, I. L., Totterdell, M. B., Banatvala, J. E.: Asymptomatic endemic rotavirus infections in the newborn. Lanceti, 1176–1178 (1978).

    Google Scholar 

  6. Flewett, T. H., Woode, G. N.: The rotaviruses. Arch. Virol.57, 1–23 (1978).

    Google Scholar 

  7. Flewett, T. H., Thouless, M. E., Pinfold, J. N., Bryden, A. S., Candeias, J. A. N.: More serotypes of human rotavirus. Lancetii, 632 (1978).

    Google Scholar 

  8. Jones, R. C., Hughes, C. S., Henry, R. R.: Rotavirus infection in commercial laying hens. Vet. Rec.104, 22 (1979).

    Google Scholar 

  9. Kapikian, A. Z., Cline, W. L., Kim, H. W., Kalica, A. R., Wyatt, R. G., Vankirk, D. H., Chanock, R. M., James, H. D., Vaughn, A. L.: Antigenic relationships among five reovirus-like (RVL) agents by complement fixation (CF) and development of new CF antigens for the human RVL agent of infantile gastroenteritis. Proc. Soc. exp. Biol. Med.152, 535–539 (1976).

    Google Scholar 

  10. McFerran, J. B., McCracken, R. M., McKillop, E. R., McNulty, M. S., Collins, D. S.: Studies on a depressed egg production syndrome in Northern Ireland. Avian Pathol.7, 35–47 (1978).

    Google Scholar 

  11. McNulty, M. S., Curran, W. L., McFerran, J. B.: The morphogenesis of a cytopathic bovine rotavirus in Madin-Darby bovine kidney cells. J. gen. Virol.33, 503–508 (1976).

    Google Scholar 

  12. McNulty, M. S., McFerran, J. B., Bryson, D. G., Logan, E. F., Curran, W. L.: Studies on rotavirus infection and diarrhoea in young calves. Vet. Rec.99, 229–230 (1976).

    Google Scholar 

  13. McNulty, M. S., Allan, G. M., Pearson, G. R., McFerran, J. B., Curran, W. L., McCracken, R. M.: Reovirus-like agent (rotavirus) from lambs. Infect. Immun.14, 1332–1338 (1976).

    Google Scholar 

  14. McNulty, M. S., Allan, G. M., McFerran, J. B.: Cell culture studies with a cytopathic bovine rotavirus. Arch. Virol.54, 201–209 (1977).

    Google Scholar 

  15. McNulty, M. S.: Rotaviruses. J. gen. Virol.40, 1–18 (1978).

    Google Scholar 

  16. McNulty, M. S., Allan, G. M., Stuart, J. C.: Rotavirus infection in avian species. Vet. Rec.103, 319–320 (1978).

    Google Scholar 

  17. Snodgrass, D. R., Wells, P. W.: The immunoprophylaxis of rotavirus infections in lambs. Vet. Rec.102, 46–48 (1978).

    Google Scholar 

  18. Thell, K. W., Bohl, E. H., Agnes, A. G.: Cell culture propagation of porcine rotavirus (reovirus-like agent). Amer. J. vet. Res.38, 1765–1768 (1977).

    Google Scholar 

  19. Thouless, M. E., Bryden, A. S., Flewett, T. H., Woode, G. N., Bridger, J. C., Snodgrass, D. R., Herring, J. A.: Serological relationships between rotaviruses from different species as studied by complement-fixation and neutralisation. Arch. Virol.53, 287–294 (1977).

    Google Scholar 

  20. Woode, G. N., Bridger, J. C., Jones, J. M., Flewett, T. H., Bryden, A. S., Davies, H. A., White, G. B. B.: Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice and foals. Infect. Immun.14, 804–810 (1976).

    Google Scholar 

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Authors and Affiliations

  1. Veterinary Research Laboratories, Stormont, Belfast, Northern Ireland

    M. S. McNulty, G. M. Allan, D. Todd & J. B. McFerran

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  1. M. S. McNulty
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  2. G. M. Allan
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  3. D. Todd
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  4. J. B. McFerran
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McNulty, M.S., Allan, G.M., Todd, D. et al. Isolation and cell culture propagation of rotaviruses from turkeys and chickens. Archives of Virology 61, 13–21 (1979). https://doi.org/10.1007/BF01320587

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  • DOI: https://doi.org/10.1007/BF01320587

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