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Structural similarities of hog cholera virus with togaviruses

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Summary

Hog cholera virus grown in PK-15 cells was purified by centrifugation through a sucrose cushion followed by sucrose gradient centrifugation. Analysis of virus labeled externally with [3H]sodium borohydride on polyacrylamide gel electrophoresis revealed two glycoproteins, gp55 and gp46. A third structural polypeptide, p36, seems not to be glycosylated. The gp46 was also found in the virus-free supernatant of infected cells. It could be demonstrated by radioimmune precipitation of virus labeled with [35S]methionine that all three polypeptides are specific for hog cholera virions. Electron microscopically hog cholera virus appeared as a spherical particle with a diameter of 42±8 nm. The virus particles frequently displayed a fringe of projections with a length of about 6–8 nm. The similarities of hog cholera virus with Alphaviruses and Flaviviruses are discussed.

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Authors and Affiliations

  1. Federal Research Institute for Animal Virus Diseases, Tübingen, Federal Republic of Germany

    P. -J. Enzmann & F. Weiland

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  1. P. -J. Enzmann
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  2. F. Weiland
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Enzmann, P.J., Weiland, F. Structural similarities of hog cholera virus with togaviruses. Archives of Virology 57, 339–348 (1978). https://doi.org/10.1007/BF01320073

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  • DOI: https://doi.org/10.1007/BF01320073

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