Summary
Fibroblasts growing on glass have microfilaments arranged in bundles. These can be demonstrated by indirect immunofluorescent technique using human antiactin serum or experimentally produced rabbit anti-actin serum. When monolayer cultures of epithelial cells and fibroblasts are infected with paramyxovirus, such as measles, mumps, Sendai and NDV, there is a striking decrease of the bundles. Rabies and adenoviruses do not seem to influence the staining of microfilaments. The microfilament decreasing effect in the cells correlates to the finding by SDS-polyacrylamide-gel-electrophoresis of actin within virions of the paramyxoviruses.
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Buckley, I., Porter, K.: Cytoplasmic fibrils in living cultured cells. A light and electron microscopic study. Protoplasma64, 349–356 (1967).
Choppin, P. W., Compans, R. W.: Reproduction of paramyxoviruses. In:Franekel-Conrat, H., Wagner, R. R. (eds.), Comprehensive Virology, Vol. 4, 95–125. New York: Plenum Press 1975.
Ehrnst, A., Sundqvist, K.-G.: Polar appearance and nonligand induced spreading of measles virus hemagglutinin at the surface of chronically infected cells. Cell5, 351–359 (1975).
Fleissner, E., Tress, E.: Chromatographic and electrophoretic analysis of viral proteins from hamster and chicken cells transformed by Rous sarcoma virus. J. Virol.11, 250–262 (1973).
Goldman, K., Knipe, D.: Functions of cytoplasmic fibers in non-muscle cell motility. Cold Spring Harbor Symp. quant. Biol.31, 529–535 (1973).
Goldman, R., Lazarides, E., Pollack, R., Weber, K.: The distribution of actin in non-muscle cells: The use of actin antibody in the localization of actin within microfilament bundles of mouse 3T3 cells. Exp. Cell Res.90, 333–344 (1975).
Howatson, A. F., Whitmore, G. F.: The development and structure of vesicular stomatitis virus. Virology16, 466–478 (1962).
Lamb, R. A., Mahy, B. W. T., Choppin, P. W.: The synthesis of Sendai virus polypeptides in infected cells. Virology69, 116–131 (1976).
Lazarides, E.: Aspects of the structural organization of actin filaments in tissue culture cells. In:Goldman, R., Pollard, T., Rosenbaum, J. (eds.), Cell Motility. Cold Spring Harbor Conferences on Cell Proliferation3, 347–360 (1976).
Lidman, K., Biberfeld, G., Fagraeus, A., Norberg, R., Torstensson, R., Utter, G., Carlsson, L., Luca, J., Lindberg, U.: Antiactin specificity of human smooth muscle antibodies in chronic active hepatitis. Clin. exp. Immunol.24, 266–272 (1976).
Matsumato, S., Kawai, A.: Comparative studies on development of rabies in different host cells. Virology39, 449–459 (1969).
McNutt, N., Culp, N., Black, P.: Revertant cell lines isolated from SV 40 transformed cells. IV. Microfilament disruption and cell shape in untransformed, transformed and revertant cells. J. Cell Biol.50, 412–418 (1973).
Pollack, R., Osborn, M., Weber, K.: Patterns of organization of actin and myosin in normal and transformed cultured cells. Proc. Nat. Acad. Sci. U.S.A.72, 994–998 (1975).
Rutter, G., Mannweller, K.: Alterations of actin-containing structures in BHK 21 cells infected with Newcastle disease virus and vesicular stomatitis virus. J. gen. Virol.37, 233–242 (1977).
Tyrrell, D. L. J., Ehrnst, A.: Transmembranal communication of measles viral proteins. A possible role for actin in viral assembly. Submitted for publication.
Tyrrell, D. L. J., Norrby, E.: Structural polypeptides of measles virus. J. gen. Virol. (in press, 1978).
Wanger, R. R., Prevec, L., Brown, F., Summers, D. F., Sokol, F., Macleod, R.: Classification of rhabdovirus proteins: a proposal. J. Virol.10, 1228–1230 (1972).
Wang, E., Wolf, B. A., Lamb, K. A., Choppin, R. W., Goldberg, A. R.: The presence of actin in enveloped viruses. In:Goldman, R., Pollard, R., Rosenbaum, J. (eds.), Cell Motility. Cold Spring Harbor Conferences on Cell Proliferation3, 589–599 (1976).
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D. L. J.Tyrrell is a Centennial Fellow of the Medical Research Council of Canada.
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Fagraeus, A., Tyrrell, D.L.J., Norberg, R. et al. Actin filaments in paramyxovirus-infected human fibroblasts studied by indirect immunofluorescence. Archives of Virology 57, 291–296 (1978). https://doi.org/10.1007/BF01320068
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DOI: https://doi.org/10.1007/BF01320068