Skip to main content
SpringerLink
Log in

Replication of baculoviruses in established insect cell lines: Phenomenon of attenuation

  • Brief Review
  • Published:
Archives of Virology Aims and scope Submit manuscript

Conclusion

The experimental data discussed above confirm that many insect cell lines are suitable for Baculoviruses, especially for the production of NPV after primary virus infection. However prolonged undiluted passagein vitro may result in virus variation and a decline of virulence for the original host.

This phenomenon is interesting as an example of virus attenuation in the system Baculovirus—in vitro cultured cell. Such phenomena must be taken into account when working with cell cultures in basic laboratory studies of the growth of Baculoviruses. It is also of practical importance, NPV variants unsuitable for insecticide production may be selected. Standard virological techniques can be applied to avoid such selection and to preserve the original characteristics of the virus population.

Further studies of Baculoviridae attenuation and host-virus interactions controlling infective processes are necessary in order to understand the mechanism of attenuation and the factors that influence the evoluation ofBaculoviridae.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Arber, W., Dussoix, D.: Host specificity of DNA produced by Escherichia coli. I. Host-controlled modification of Bacteriophage λ. J. mol. Biol.5, 18–36 (1962).

    Google Scholar 

  2. Bergold, G. H.: The nature of nuclear polyhedrosis virus. In:Steinhaus, E. A. (ed.), Insect Pathology1, 417–456. New York: Academic Press 1963.

    Google Scholar 

  3. Brooks, M. A., Kurtti, T. J.: Insect cell and tissue culture. Ann. Rev. Entomol.16, 27–52 (1971).

    Google Scholar 

  4. Brown, M., Faulkner, P.: Factors affecting the yield of virus in a cloned cell line ofTrichoplusia ni infected with a nuclear polyhedrosis virus. J. invertebr. Pathol.26, 251–257 (1975).

    Google Scholar 

  5. Dougherty, E. M., Vaughn, J. L., Reichelderfer, Ch. F.: Characteristics of the non-occluded form of a nuclear polyhedrosis virus. Intervirology5, 109–121 (1975).

    Google Scholar 

  6. Faulkner, P., Henderson, J. F.: Serial passage of a nuclear polyhedrosis disease virus of the cabbage looper(Trichoplusia ni) in a continuous tissue culture cell line. Virology50, 920–924 (1972).

    Google Scholar 

  7. Gardiner, G. R., Stockdale, H.: Two tissue culture media for production of lepidopteran cells and nuclear polyhedrosis viruses. J. Invertebrate Pathol.25, 363–370 (1975).

    Google Scholar 

  8. Gerschenson, S. M.: On the species-specificity of viruses causing nuclear poly-hedrosis diseases of insects. Microbiologia24, 90–98 (1956).

    Google Scholar 

  9. Ghendon, Ju. Z.: Genetics of viruses infecting man and animals. Moscow: Meditsina 1967.

    Google Scholar 

  10. Ghendon, Ju. Z., Poglazov, B. F., Tikchonenko, T. I.: Nucleic acids and proteins of virions. Moscow: Nauka 1975.

    Google Scholar 

  11. Goodwin, K. H., Vaughn, J. F., Louloudes, S. J., Adams, J. R.: Replication of a nuclear polyhedrosis virus in an established insect cell line. J. Invertebrate Pathol.16, 284–288 (1970).

    Google Scholar 

  12. Grace, T. D. C.: Insect tissue culture and its use in virus research. Adv. Virus Res.14, 201–220 (1969).

    Google Scholar 

  13. Grace, T. D. C.: Cultured cells in virus research. In:Gibbs, A. J. (ed.), Viruses and invertebrates, 321–348. Amsterdam-London-New York: North Holland Rebl. Co. American Elsevier Publ. Co., Inc. 1973.

    Google Scholar 

  14. Granados, R. R.: Infection and replication of insect pathogenic viruses in tissue culture. Adv. Virus Res.20, 189–236 (1976).

    Google Scholar 

  15. Harrap, K. A.: Cell infection by a nuclear polyhedrosis virus. Virology42, 311–318 (1970).

    Google Scholar 

  16. Henderson, J. F., Faulkner, P., Mac Kinnon, E. A.: Some biophysical properties of virus present in tissue cultures infected with the nuclear polyhedrosis virus ofTrichoplusia ni. J. gen. Virol.22, 143–146 (1974).

    Google Scholar 

  17. Hink, W. F., Strauss, E.: Replication and passage of alfalfa looper nuclear polyhedrosis virus plaque variants in cloned cell cultures and larval stages of four host species. J. Invertebrate Pathol.27, 49–55 (1976).

    Google Scholar 

  18. Hink, W. F., Vail, P. V.: A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus in a cabbage looper (TN 368) cell line. J. Invertebrate Pathol.22, 168–174 (1973).

    Google Scholar 

  19. Ignoffo, C. M.: Viruses-living insecticides. In: Current Topics in Microbiology and Immunology42, 139–167. Berlin-Heidelberg-New York: Springer 1968.

    Google Scholar 

  20. Ignoffo, C. M., Hostetter, D. L., Shapiro, M.: Efficacy of insect viruses propagatedin vivo andin vitro. J. Invertebrate Pathol.24, 184–187 (1974).

    Google Scholar 

  21. Kelly, D. C.: “Oryctes” virus replication: electron microscopic observations on infected moth and mosquite cells. Virology69, 596–606 (1976).

    Google Scholar 

  22. Knudson, D. L., Harrap, K. A.: Replication of a nuclear polyhedrosis virus in a continuous cell culture ofSpodoptera frugiperda. Microscopy study of the sequence of events of virus infection. J. Virol.17, 254–268 (1976).

    Google Scholar 

  23. Knudson, D. L., Tinsley, T. W.: Replication of a nuclear polyhedrosis virus in a continuous cell culture ofSpodoptera frugiperda: purification, assay of infectivity, and growth characteristics of the virus. J. Virol.14, 934–944 (1974).

    Google Scholar 

  24. Lwoff, A., Tournier, P.: Remarks on the classification of viruses. In:Maramorosch, K., Kurstak, E. (eds.), Comparative Virology, 1–42. New York-London: Academic Press 1971.

    Google Scholar 

  25. MacKinnon, E. A., Henderson, J. F., Stoltz, D. B., Faulkner, P.: Morphogenesis of nuclear polyhedrosis virus under condition of prolonged passagein vitro. J. ultrastruct. Res.49, 419–435 (1974).

    Google Scholar 

  26. Mayr, E.: Populations, species and evolution. Cambridge, Massachusetts: The Belknap Press of Harvard University Press 1970.

    Google Scholar 

  27. McIntosh, A. H.:In vitro specificity and mechanism of infection. In: Baculoviruses for insect pest control: safety considerations, 63–69. American Society for Microbiology 1975.

  28. Miltenbürger, H. G., David, P.: Nuclear polyhedrosis virus replication in permanent cell lines of the cabbage moth(Mamestra brassicae L.). Naturwissensch.63, 197–198 (1976).

    Google Scholar 

  29. Potter, K. N., Faulkner, P., MacKinnon, E. A.: Strain selection during serial passage ofTrichoplusia ni nuclear polyhedrosis virus. J. Virol.18, 1040–1050 (1976).

    Google Scholar 

  30. Pshenitchnov, W. A., Vorobiov, A. A., Balayeva, N. M.: Attenuation of viruses and ricketsiae. Moscow: Meditsina 1976.

    Google Scholar 

  31. Raghow, P., Grace, T. D. C.: Studies on a nuclear polyhedrosis virus inBombyx mori cellsin vitro. J. ultrastruct. Res.47, 384–399 (1974).

    Google Scholar 

  32. Ramoska, W. A., Hink, W. F.: Electron microscope examination of two plaque variants from a nuclear polyhedrosis virus of the alfalfa looper,Autographa californica. J. Invertebrate Pathol.23, 197–201 (1974).

    Google Scholar 

  33. Sohi, S. S., Cunningham, J. C.: Replication of a nuclear polyhedrosis virus in serially transferred insect hemocyte culture. J. Invertebrate Pathol.19, 51–61 (1972).

    Google Scholar 

  34. Vago, C., Bergoin, M.: Viruses in invertebrates. Adv. Virus Res.13, 248–304 (1969).

    Google Scholar 

  35. Vail, P. V., Jay, D. L., Hink, W. F.: Replication and infectivity of the nuclear polyhedrosis virus of the alfalfa looper,Autographa californica, produced in cells grownin vitro. J. Invertebrate Pathol.22, 231–237 (1973).

    Google Scholar 

  36. Vail, P. V., Jay, D. L., Romine, C. L.: Replication of theAutographa californica nuclear polyhedrosis virus in insect cell lines grown in modified media. J. Invertebrate Pathol.28, 263–267 (1976).

    Google Scholar 

  37. Vaughn, J. V., Faulkner, P.: Susceptibility of an insect tissue culture to infection by virus preparations of the nuclear polyhedrosis of the silkworm(Bombyx mori). Virology20, 484–489 (1963).

    Google Scholar 

  38. Volkman, L. E., Summers, M. D.: Nuclear polyhedrosis virus detection: relative capabilities of clones developed fromTrichoplusia ni ovarian cells in plaque assay. J. Virol.16, 1630–1637 (1975).

    Google Scholar 

  39. Witt, R. J., Janus, Ch. A.: Replication ofGalleria mellonella nuclear polyhedrosis virus in cultured cells and in larvae ofTrichoplusia ni. J. Invertebrate Pathol.29, 222–226 (1977).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular Biology and Genetics, Academy of Sciences of the Ukrainian S.S.R., Kiev, USSR

    E. N. Zherebtsova

Authors
  1. E. N. Zherebtsova
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zherebtsova, E.N. Replication of baculoviruses in established insect cell lines: Phenomenon of attenuation. Archives of Virology 57, 283–290 (1978). https://doi.org/10.1007/BF01320067

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01320067

Keywords

Search

Navigation