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Inhibition of photosynthesis by ultraviolet radiation as a function of dose and dosage rate: Results for a marine diatom

Marine Biology volume 111, pages 183–190 (1991)Cite this article

Abstract

The effects of ultraviolet radiation on phytoplankton are usually described as a function of dose (J m−2, weighted appropriately). Experiments conducted in 1988 and 1989 on a marine diatom,Thalassiosira pseudonana (Clone 3H), demonstrate that during lightlimited photosynthesis in visible radiation, the inhibition of photosynthesis by supplemental ultraviolet radiation (principally UV-B: 280 to 320 nm) is a function of irradiance (W m−2) as well as of dose: for equal doses of UV-B, a relatively short exposure to high UV-B irradiance is more damaging to photosynthesis than a longer exposure to lower irradiance. In fact, photoinhibition by UV-B is well described as a monotonic, nonlinear function of irradiance for time scales of 0.5 to 4 h. A nitrate-limited culture was about nine times more sensitive to UV-B than was a nutrient-replete culture, but the kinetics of photoinhibition were similar. These results have some bearing on efforts to describe the effects of ultraviolet radiation on marine primary productivity. Action spectra of photoinhibition by UV can be constructed, but they should only be used to describe photoinhibition for specified time scales. Vertical profiles of relative photoinhibition must be interpreted cautiously because photoinhibition by UV-B is likely to be a function of incubation time and results must therefore be interpreted in the context of vertical mixing.

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Literature cited

  • Caldwell, M. M. (1971). Solar ultraviolet radiation and the growth and development of higher plants. In: Giese, A. C. (ed.) Photophysiology. Academic Press, New York, p. 131–177

    Google Scholar 

  • Caldwell, M. M., Camp, L. B., Warner, C. W., Flint, S. D. (1986). Action spectra and their key role in assessing biological consequences of solar UV-B radiation change. In: Worrest, R. C., Caldwell, M. M. (eds.) Stratospheric ozone reduction, solar ultraviolet radiation and plant life. Springer, New York, p. 87–111

    Google Scholar 

  • Calkins, J., Thordardottir, T. (1980). The ecological significance of solar UV radiation on aquatic organisms. Nature, Lond. 283: 563–566

    Google Scholar 

  • Cleveland, J. S., Perry, M. J. (1987). Quantum yield, relative specific absorption and fluorescence in nitrogen-limitedChaetoceros gracilis. Mar. Biol. 94: 489–497

    Google Scholar 

  • Coohill, T. P. (1989). Ultraviolet action spectra (280 to 380 nm) and solar effectiveness spectra for higher plants. Photochem. Photobiol. 50: 451–457

    Google Scholar 

  • Cullen, J. J. (1990). On models of growth and photosynthesis in phytoplankton. Deep-Sea Res. 37: 667–683

    Google Scholar 

  • Döhler, G., Biermann, I. (1987). Effect of u.v.-B irradiance on the response of15N-nitrate uptake ofLauderia annulata andSynedra planctonica. J. Plankton Res. 9: 881–890

    Google Scholar 

  • Gallegos, C. L., Platt, T. (1985). Vertical advection of phytoplankton and productivity estimates: a dimension analysis. Mar. Ecol. Prog. Ser. 26: 125–134

    Google Scholar 

  • Guillard, R. R. L. (1975). Culture of phytoplankton for feeding marine invertebrates. In: Smith, W. L., Chanley, M. H. (eds.) Culture of marine invertebrate animals. Plenum Press, New York, p. 29–60

    Google Scholar 

  • Häder, D.-P., Häder, M. (1988). Inhibition of motility and phototaxis in the green flagellate,Euglena gracilis, by UV-B radiation. Archs Microbiol. 150: 20–25

    Google Scholar 

  • Harris, G. P. (1980). Spatial and temporal scales in phytoplankton ecology. Mechanisms, methods, models and management. Can. J. Fish. aquat. Sciences 37: 877–900

    Google Scholar 

  • Hirosawa, T., Miyachi, M. (1983). Inactivation of Hill reaction by long-wavelength radiation (UV-A) and its photoreactivation by visible light in the cyanobacterium,Anacystis nidulans. Archs. Microbiol. 135: 98–102

    Google Scholar 

  • Jokiel, P. L., York, J. R. H. (1984). Importance of ultraviolet radiation in photoinhibition of microalgal growth. Limnol. Oceanogr. 29: 192–199

    Google Scholar 

  • Jones, L. W., Kok, B. (1966). Photoinhibition of chloroplast reactions. I. Kinetics and action spectra. Pl. Physiol., Lancaster 41: 1037–1043

    Google Scholar 

  • Kiefer, D. A. (1973). Chlorophylla fluorescence in marine diatoms: responses of chloroplasts to light and nutrient stress. Mar. Biol. 23: 39–46

    Google Scholar 

  • Kok, B. (1956). On the inhibition of photosynthesis by intense light. Biochim. biophys. Acta 21: 234–244

    Google Scholar 

  • Kullenberg, G. (1982). Note on the role of vertical mixing in relation to effects of UV radiation on the marine environment. In: Calkins, J. (ed.) The role of solar UV radiation in marine ecosystems. Plenum Press, New York, p. 283–292

    Google Scholar 

  • Kyle, D. J. (1987). The biochemical basis for photoinhibition of photosystem II. In: Kyle, D. J., Osmond, C. B., Arntzen, C. J. (eds.) Photoinhibition. Elsevier, Amsterdam, p. 197–226

    Google Scholar 

  • Legendre, L., Demers, S. (1984). Towards dynamic biological oceanography and limnology. Can. J. Fish. aquat. Sciences 41: 2–19

    Google Scholar 

  • Lesser, M. P., Shick, J. M. (1989). Effects of irradiance and ultraviolet radiation on photoadaptation in the zooxanthellae ofAiptasia pallida: primary production, photoinhibition, and enzymic defenses against oxygen toxicity. Mar. Biol. 102: 243–255

    Google Scholar 

  • Lewis, M. R., Cullen, J. J., Platt, T. (1984). Relationships between vertical mixing and photoadaptation of phytoplankton: similarity criteria. Mar. Ecol. Prog. Ser. 15: 141–149

    Google Scholar 

  • Marra, J. (1978a). Effect of short-term variation in light intensity on photosynthesis of a marine phytoplankter: a laboratory simulation study. Mar. Biol. 46: 191–202

    Google Scholar 

  • Marra, J. (1978b). Phytoplankton photosynthetic response to vertical movement in a mixed layer. Mar. Biol. 46: 203–208

    Google Scholar 

  • Maske, H. (1984). Daylight ultraviolet radiation and the photoinhibition of phytoplankton carbon uptake. J. Plankton Res. 6: 351–357

    Google Scholar 

  • Neale, P. J. (1987). Algal photoinhibition and photosynthesis in the aquatic environment. In: Kyle, D. J., Osmond, C. B., Arntzen, C. J. (eds.) Photoinhibition. Elsevier, Amsterdam, p. 35–65

    Google Scholar 

  • Neale, P. J., Richerson, P. J. (1987). Photoinhibition and the diurnal variation of phytoplankton photosynthesis — I. Development of a photosynthesis-irradiance model from studies of in situ responses. J. Plankton Res. 9: 167–193

    Google Scholar 

  • Ohad, I., Kyle, D. J., Arntzen, C. J. (1984). Membrane protein damage and repair: removal and replacement of inactivated 32-kilodalton polypeptides in chloroplast membranes. J. Cell Biol. 99: 481–485

    Google Scholar 

  • Prézelin, B. B., Samuelsson, G., Matlick, H. A. (1986). Photosystem II photoinhibition and altered kinetics of photosynthesis during nutrient-dependent high-light photoadaptation inGonyaulax polyedra. Mar. Biol. 93: 1–12

    Google Scholar 

  • Redford, E., Myers, J. (1951). Some effects of UV radiation on the metabolism ofChlorella. J. cell. comp. Physiol. 38: 217–243

    Google Scholar 

  • Richter, M., Rühle, W., Wild, A. (1990a). Studies on the mechanism of photosystem II photoinhibition I. A two-step degradation of D1 protein. Photosynth. Res. 24: 229–235

    Google Scholar 

  • Richter, M., Rühle, W., Wild, A. (1990b). Studies on the mechanism of photosystem II photoinhibition II. The involvement of toxic oxygen species Photosynthesis. Res. 24: 237–243

    Google Scholar 

  • Samuelsson, G., Lönneborg, A., Rosenqvist, E., Gustafson, P., Öquist, G. (1985). Photoinhibition and reactivation of photosynthesis in the cyanobacteriumAnacystis nidulans. Pl. Physiol. 79: 992–995

    Google Scholar 

  • Setlow, R. B. (1974). The wavelengths in sunlight effective in producing skin cancer: a theoretical analysis. Proc. natn. Acad. Sci. U.S.A. 71: 3363–3366

    Google Scholar 

  • Sisson, W. B. (1986). Effects of UV-B radiation on photosynthesis. In: Worrest, R. C., Caldwell, M. M. (eds.) Stratospheric ozone reduction, solar ultraviolet radiation and plant life. Springer, New York, p. 161–169

    Google Scholar 

  • Smith, R. C. (1989). Ozone, middle ultraviolet radiation and the aquatic environment. Photochem. Photobiol. 50: 459–468

    Google Scholar 

  • Smith, R. C., Baker, K. S. (1982). Assessment of the influence of enhanced UV-B on marine primary productivity. In: Calkins, J. (ed.) The role of solar ultraviolet radiation in marine ecosystems. Plenum Press, New York, p. 509–537

    Google Scholar 

  • Smith, R. C., Baker, K. S. (1989). Stratospheric ozone, middle ultraviolet radiation and phytoplankton productivity. Oceanogr. Mag. 2: 4–10

    Google Scholar 

  • Smith, R. C., Baker, K. S., Holm-Hansen, O., Olson, R. S. (1980). Photoinhibition of photosynthesis in natural waters. Photochem. Photobiol. 31: 585–592

    Google Scholar 

  • Trocine, R. P., Rice, J. D., Wells, G. N. (1981). Inhibition of seagrass photosynthesis by ultraviolet-B radiation. Pl. Physiol. 68: 74–81

    Google Scholar 

  • Van Baalen, C. (1968). The effects of ultraviolet irradiation on a coccoid blue-green alga: survival, photosynthesis, and photoreactivation. Pl. Physiol. 43: 1689–1695

    Google Scholar 

  • Vincent, W. F., Neale, P. J., Richerson, P. J. (1984). Photoinhibition: algal responses to bright light during diel stratification and mixing in a tropical alpine lake. J. Phycol. 20: 201–211

    Google Scholar 

  • Welschmeyer, N. A., Lorenzen, C. J. (1981). Chlorophyll-specific photosynthesis and quantum efficiency at subsaturating light intensities. J. Phycol. 17: 283–293

    Google Scholar 

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Author notes

  1. J. J. Cullen

    Present address: Department of Oceanography, Dalhousie University, B3H 4J1, Halifax, Nova Scotia, Canada

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  1. Bigelow Laboratory for Ocean Sciences, McKown Point, 04575, West Boothbay Harbor, Maine, USA

    J. J. Cullen & M. P. Lesser

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  1. J. J. Cullen
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  2. M. P. Lesser
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Communicated by R. O'Dor, Halifax

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Cullen, J.J., Lesser, M.P. Inhibition of photosynthesis by ultraviolet radiation as a function of dose and dosage rate: Results for a marine diatom. Mar. Biol. 111, 183–190 (1991). https://doi.org/10.1007/BF01319699

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