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Deduced amino acid sequences at the haemagglutinin cleavage site of avian influenza A viruses of H 5 and H 7 subtypes

Archives of Virology volume 130, pages 209–217 (1993)Cite this article

Summary

The amino acid sequences at the haemagglutinin cleavage sites of 9 avian influenza A viruses of H 5 subtype (5 high and 4 low pathogenicity for chickens) and 21 of H 7 subtype (13 high and 8 low pathogenicity for chickens) were determined by direct RNA sequencing, PCR amplification sequencing or both. None of the viruses of low pathogenicity had multiple basic amino acids at the cleavage site. All highly pathogenic viruses had an insert of basic amino acids at the cleavage site, except A/chicken/Scotland/59 (H5N1) for which the multiple basic amino acids appeared as substitutions and not insertions. All highly pathogenic viruses examined conformed to the amino acid motif of R-X-R/K-R at the cleavage site which is considered to be essential for high pathogenicity in chickens, with the notable exception of highly pathogenic virus A/turkey/England/50-92/91 (H5N1) which had the sequence R-K-R-K-T-R adjacent to the cleavage site.

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Affiliations

  1. Avian Virology, Central Veterinary Laboratory, Weybridge, Surrey

    G. W. Wood, J. B. Bashiruddin & D. J. Alexander

  2. Division of Molecular Biology, AFRC Institute for Animal Health, Pirbright, Surrey, United Kingdom

    J. W. McCauley

Authors
  1. G. W. Wood
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  2. J. W. McCauley
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  3. J. B. Bashiruddin
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  4. D. J. Alexander
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Wood, G.W., McCauley, J.W., Bashiruddin, J.B. et al. Deduced amino acid sequences at the haemagglutinin cleavage site of avian influenza A viruses of H 5 and H 7 subtypes. Archives of Virology 130, 209–217 (1993). https://doi.org/10.1007/BF01319010

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  • DOI: https://doi.org/10.1007/BF01319010

Keywords

  • Infectious Disease
  • Amino Acid Sequence
  • Influenza
  • Cleavage Site
  • Deduce Amino Acid Sequence