Summary
The human Wa strain of rotaviruses, initially unable to grow in liver cells, was adapted by multiple passages to grow in HepG2 cells. The genome segment 4 of both the parental and passaged strains was cloned and sequenced. Five amino acid differences (residues 38, 120, 421, 525, and 618) were found in the HepG2-passaged variant compared to the parental Wa strain. Our results support the hypothesis that viral variants that have improved capabilities for infecting liver cells can be generated during infection.
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Kitamoto, N., Mattion, N.M. & Estes, M.K. Alterations in the sequence of the gene 4 from a human rotavirus after multiple passages in HepG2 liver cells. Archives of Virology 130, 179–185 (1993). https://doi.org/10.1007/BF01319006
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DOI: https://doi.org/10.1007/BF01319006