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Ultrastructural studies on a chlorella virus from Germany

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Summary

This is the first report on the morphology and fine structure of chlorella virus Göttingen-1 (CVG-1), an European member of the newly approved familyPhycodnaviridae, which infects certain unicellular, eukaryotic, exsymbiotic, green algae. CVG-1 are polyhedral particles 145–160 nm in diameter. The capsid consists of two shells, apparently composed of ca. 7 nm subunits. Whereas the outer shell of the capsid appears to be icosahedral, the inner shell appears to be irregular underneath one vertex of the virion. In this vertex the inner shell is separated from the outer shell leaving a distinct space (“empty vertex”) of unknown function.

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References

  1. Becker B (1991) Untersuchungen zur Charakterisierung vonChlorella-Viren. Hartung-Gorre Verlag, Konstanz

    Google Scholar 

  2. Dawe VH and Kuhn CW (1983) Virus-like particles in the aquatic fungus,Rhizidiomyces. Virology 130: 10–20

    Google Scholar 

  3. Devauchelle G, Stoltz DB, Darcy-Tripier F (1985) Comparative ultrastructure of Iridoviridae. In: Willis DB (ed) Iridoviridae. Springer, Berlin Heidelberg New York Tokyo, pp 1–21 (Current topics in microbiology and immunology, vol 116)

    Google Scholar 

  4. Dodds JA (1979) Viruses of marine algae. Experientia 35: 440–442

    Google Scholar 

  5. Dodds JA (1983) New viruses of eukaryotic algae and protozoa. In: Matthews REF (ed) A critical appraisal of viral taxonomy. CRC Press, Boca Raton, pp 177–188

    Google Scholar 

  6. Francki RIB, Fauquet CM, Knudson DL, Brown F (eds) (1991) Classification and nomenclature of viruses. Springer, Wien New York (Arch Virol [Suppl] 2)

    Google Scholar 

  7. Kawakami H, Kawakami N (1978) Behavior of a virus in a symbiotic system,Paramecium bursaria-Zoochlorella. J Protozool 25: 217–225

    Google Scholar 

  8. Kelly DC, Vance DE (1973) The lipid content of two iridescent viruses. J Gen Virol 21: 417–423

    Google Scholar 

  9. Kelly DC (1985) Insect iridescent viruses. In: Willis DB (ed) Iridoviridae. Springer, Berlin Heidelberg New York Tokyo, pp 23–31 (Current topics in microbiology and immunology, vol 116)

    Google Scholar 

  10. Koenig R, Lesemann DE (1985) Plant viruses in German rivers and lakes. I. Tombusviruses, a potexvirus and carnation mottle virus. Phytopathol Z 112: 1105–1116

    Google Scholar 

  11. Lee RE (1971) Systemic viral material in the cells of the freshwater red algaSirodotia tenuissima (Holden) Skuja. J Cell Sci 8: 623–631

    Google Scholar 

  12. Lemke PA (1976) Viruses of eukaryotic microorganisms. Annu Rev Microbiol 30: 105–145

    Google Scholar 

  13. Manyakov VF (1977) Fine structure of the iridescent virus type I capsid. J Gen Virol 36: 73–79

    Google Scholar 

  14. Meints RH, Schuster AM, Van Etten JL (1985)Chlorella viruses. Plant Mol Biol Rep 3: 180–187

    Google Scholar 

  15. Meints RH, Burbank DE, Van Etten JL, Lamport DTA (1988) Properties of theChlorella receptor for the virus PBCV-1. Virology 164: 15–21

    Google Scholar 

  16. Pan IC, Murakami Y, Saiz JC (1990) African swine fever virus: purification of capsomeres released by lipase. Virology 175: 271–273

    Google Scholar 

  17. Reisser W, Becker B, Klein T (1986) Studies on ultrastructure and host range of aChlorella attacking virus. Protoplasma 135: 162–165

    Google Scholar 

  18. Reisser W, Klein T, Becker B (1988) Studies on phycoviruses. I. On the ecology of viruses attackingChlorellae exsymbiotic from an European strain ofParamecium bursaria. Arch Hydrobiol 111: 575–583

    Google Scholar 

  19. Reisser W, Kapaun E (1991) Entry of aChlorella virus in its host cell. J Phycol 27: 609–613

    Google Scholar 

  20. Reisser W (1992) Phycoviruses. Arch Protistenkd 92 (in press)

  21. Rohozinski J, Girton LE, Van Etten JL (1989)Chlorella viruses contain linear non-permuted double-stranded DNA genomes with covalently closed hairpin ends. Virology 168: 363–369

    Google Scholar 

  22. Ryter A, Kellenberger E, Birch-Andersen A, Maaloe (1958) Etude au microscope électronique de plasmas contenant de l'acide désoxyribonucléique. I. Les nucléoides des bactéries an croissance active. Z Naturforsch 136: 597–605

    Google Scholar 

  23. Sherman LA, Brown RM (1978) Cyanophages and viruses of eukaryotic algae. In: Fraenkel-Conrat H, Wagner RR (eds) Comprehensive virology, vol 12. Plenum, New York, pp 145–233

    Google Scholar 

  24. Skrdla MP, Burbank DE, Xia Y, Meints R, van Etten JL (1984) Structural proteins and lipids in a virus, PBCV-1 which replicates in aChlorella-like alga. Virology 135: 308–315

    Google Scholar 

  25. Stoltz DB (1971) The structure of icosahedral cytoplasmatic desoxyriboviruses. J Ultrastruct Res 37: 219–239

    Google Scholar 

  26. Van Etten JL, Meints RH, Kuczmarski D, Burbank DE, Lee K (1982) Viruses of symbioticChlorella-like algae isolated fromParamecium bursaria andHydra viridis. Proc Natl Acad Sci USA 79: 3867–3871

    Google Scholar 

  27. Van Etten JL, Burbank DE, Kuczmarski D, Meints RH (1983) Virus infection of culturableChlorella-like algae and development of a plaque assay. Science 219: 994–996

    Google Scholar 

  28. Van Etten JL, Lane LC, Meints RH (1991) Unicellular plants also have large dsDNA viruses. Semin Virol 2: 71–77

    Google Scholar 

  29. Van Etten JL, Lane LC, Meints RH (1991) Viruses and viruslike particles of eukaryotic algae. Microbiol Rev 55: 586–620

    Google Scholar 

  30. Wrigley NG (1969) An electron microscope study of the structure ofSericestis iridescent virus. J Gen Virol 5: 123–134

    Google Scholar 

  31. Yamashita S, Doi Y, Yora K (1973) Intracellular appearance ofPenicillium chrysogenum virus. Virology 55: 445–452

    Google Scholar 

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Becker, B., Lesemann, D.E. & Reisser, W. Ultrastructural studies on a chlorella virus from Germany. Archives of Virology 130, 145–155 (1993). https://doi.org/10.1007/BF01319003

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