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Plaque assay ofHeliothis zea baculovirus employing a mixed agarose overlay

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Summary

The nuclear polyhedrosis virus ofHeliothis zea has been titrated inHeliothis zea cells by the plaque method, using 1 percent mixed agarose containing a mixture of Seakem and Ultra pure agarose. Visible plaques, formed 8 days postinfection, ranged in diameter from 0.5 to 2 mm. Dose-response experiments indicated that a single particle initiated the formation of a plaque. The titration ofHeliothis zea baculovirus by the newly described plaque method provides an accurate technique for the determination of virus concentration.

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Authors and Affiliations

  1. Waksman Institute of Microbiology, Rutgers University, Piscataway, New Jersey, USA

    K. Yamada & K. Maramorosch

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  1. K. Yamada
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  2. K. Maramorosch
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Yamada, K., Maramorosch, K. Plaque assay ofHeliothis zea baculovirus employing a mixed agarose overlay. Archives of Virology 67, 187–189 (1981). https://doi.org/10.1007/BF01318604

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  • DOI: https://doi.org/10.1007/BF01318604

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