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Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis

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Summary

The nucleoprotein (NP) gene from avian influenza strain A/Shearwater/Aust/1/72 (H6N5) was cloned, sequenced, and expressed in vaccinia virus for the production of potent sera in immunised rabbits. The NP gene is 1565 bp and shares >95% amino acid sequence identity with other NPs of the avian subtype. The recombinant NP expressed by vaccinia virus comigrated with endogenous A/Shearwater/Aust/1/72 NP by Western blot analysis. Polyclonal rabbit sera raised against recombinant NP was evaluated in an antigen capture ELISA system as a potential diagnostic tool for the detection of avian influenza. All type A strains, comprising several HA and NA subtypes, but not type B nor other avian viruses, were detected.

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Authors and Affiliations

  1. St. Vincent's Institute of Medical Research, Fitzroy

    V. R. Harley

  2. CSIRO Division of Biotechnology, Parkville

    P. J. Hudson

  3. CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia

    B. E. H. Coupar, P. W. Selleck, H. Westbury & D. B. Boyle

Authors
  1. V. R. Harley
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  2. P. J. Hudson
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  3. B. E. H. Coupar
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  4. P. W. Selleck
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  5. H. Westbury
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  6. D. B. Boyle
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Harley, V.R., Hudson, P.J., Coupar, B.E.H. et al. Vaccinia virus expression and sequence of an avian influenza nucleoprotein gene: potential use in diagnosis. Archives of Virology 113, 133–141 (1990). https://doi.org/10.1007/BF01318362

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  • DOI: https://doi.org/10.1007/BF01318362

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