Summary
Slow-reacting, complement-requiring hemagglutination-inhibiting (HI) antibody was detected in sera from pigs infected with pseudorabies virus; approximately 16 hemolytic units of complement were necessary for the detection of such antibody. Higher HI antibody titers were obtained when antigen and serum were allowed to incubate before addition of complement than when all three components were incubated at the same time. A HI test was developed in which antigen-serum mixtures were incubated at 4°C for 48 h and then with complement at 37°C for 1 h; this gave an improved sensitivity over the previous incubation without complement.
This is a preview of subscription content, log in via an institution to check access.
References
Baughman RH, Fenters JD, Marquis Jr GS, Holper JC (1968) Effect of complement and viral filtration on the neutralization of respiratory syncytial virus. Appl Microbiol 16: 1076–1080
Bitsch V, Eshildsen M (1982) Complement-dependent neutralisation of Aujeszky's disease virus by antibody. Curr Top Vet Med Anim Sci 17: 41–49
Briaire J, Meloen RH, Barteling JS (1979) An enzyme-linked immunosorbent assay (ELISA) for detection of antibody against Aujeszky's disease virus in pig sera. Zentralbl Veterinarmed [B] 26: 76–81
Forschner E, Dopatka HD, Buenger I, Behrens F, Witte KH (1981) Aujeszkysche Krankheit des Schweines. Zur Einsatzmöglichkeit eines ELISAs für den Nachweis von virusspezifischen Serumantikörpern. Dtsch Tierärztl Wochenschr 88: 134–139
Fukusho A, Shimizu M, Kubo M, Namba K, Shimizu Y, Konno S, Suzaki K, Otaki T (1981) The first outbreak of Aujeszky's disease in swine in Japan. II. Virus isolation. Bull Natl Inst Anim Health No. 82: 5–11 (in Japanese with English summary)
Graham BJ, Minamishima Y, Dreesman GC, Haines HG, Benyesh-Melnick M (1971) Complement-requiring neutralizing antibodies in hyperimmune sera to human cytomegaloviruses. J Immunol 107: 1618–1630
Hyllseth B, Pettersson U (1970) Neutralization of equine arteritis virus: enhancing effect of guinea pig serum. Arch Ges Virusforsch 32: 337–347
Iwasaki T, Ogawa R (1968) Studies on complement-potentiated neutralizing antibodies (C'-PNAb) induced in rabbits inoculated with Japanese encephalitis virus (JEV). I. The nature of C'-PNAb. Virology 34: 46–59
Kono M (1970) Potentiating-factor dependency and molecular size of neutralizing antibodies produced in bovine parainfluenza 3 virus infection. Natl Inst Anim Health Q (Jpn) 10: 129–136
Moenning V, Woldesenbet P, Frey HR, Loess B, Dopatka HD, Behrens F (1982) Comparative evaluation of ELISA and neutralization test for the diagnosis of Aujeszky's disease. Curr Top Vet Med Anim Sci 17: 51–56
Moutou F, Toma G, Fortier B (1978) Application of an enzyme-linked immunosorbent assay for diagnosis of Aujeszky's disease in swine. Vet Rec 103: 264
Nishimura C, Nomura M, Kitaoka M, Takeuchi Y, Kimura M (1968) Complement requirement of the neutralizating antibody appearing after immunization with smallpox vaccine. Jpn J Microbiol 12: 256–259
Rawls WE, Desmyter J, Melnick JL (1967) Rubella virus neutralization by plaque reduction. Proc Soc Exp Biol Med 124: 167–172
Reno PW, Hoffman EM (1972) Enhancement of hemagglutination-inhibition by complement. Infect Immun 6: 945–951
Rossi CR, Kiesel GK (1974) Complement-requiring neutralizing antibodies in cattle to infectious bovine rhinotracheitis virus. Arch Ges Virusforsch 45: 328–334
Sato K, Tanaka Y, Kurogi H, Tokuhisa S, Namba K, Inaba Y, Matumoto M (1988) Detection of antibody to pseudorabies virus in swine sera by indirect immunoperoxidase plaque staining. J Clin Microbiol 26: 79–81
Sato K, Tanaka Y, Tokuhisa S, Inaba Y, Fushiki T, Matumoto M (1987) Appearance of slow-reacting and complement-requiring neutralizing antibody in cattle infected with Akabane virus. Vet Microbiol 14: 183–189
Shope RE (1935) Experiments on the epidemiology of pseudorabies. I. Model of transmission in swine and their possible role in its spread to cattle. J Exp Med 62: 85–99
Smith PC, Mengeling WL (1979) A skin test for pseudorabies virus infection (Aujeszky's disease) in swine. Can J Comp Med 41: 364–368
Stevens DA, Pincus T, Burroughs MAK, Hampar B (1968) Serologic relationship of a simian herpes virus (SA8) and herpes simplex virus: heterogeneity in the degree of reciprocal cross-reactivity shown by rabbit 7S and 19S antibodies. J Immunol 101: 979–983
Tetsu N, Inaba Y, Yukawa M, Ohba S, Yoshiki K, Hirahara T, Izumida A, Furuya Y, Ito S (1989) An improved hemagglutination-inhibition test for pseudorabies virus. Jpn J Vet Sci 51: 1271–1274
Tetsu N, Inaba Y, Yukawa M, Yoshiki K, Hirahara T, Furuya Y, Ito S, Yonemochi A, Ishikawa H (1989) Hemagglutination with pseudorabies virus. Arch Virol 106: 321–326
Toma B (1982) Serological diagnosis of Aujeszky's disease using enzyme-linked immunosorbent assay (ELISA). Curr Top Vet Med Anim Sci 17: 65–74
Westaway EG (1965) The neutralization of arboviruses II. Neutralization in heterologous virus-serum mixtures with four group B arboviruses. Virology 26: 528–537
Yoshino K, Taniguchi S (1964) The appearance of complement-requiring neutralizing antibodies by immunization and infection with herpes simplex virus. Virology 22: 193–201
Yoshino K, Morishima T, Aoki Y (1971) Absence of an all-or-none type of complement requirement in early neutralizing antibody against Western equine encephalitis virus. Jpn J Microbiol 15: 63–72
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Inaba, Y., Tetsu, N., Toriyoshi, H. et al. Appearance of slow-reacting, complement-requiring hemagglutination-inhibiting antibody in swine infected with pseudorabies virus. Archives of Virology 113, 115–124 (1990). https://doi.org/10.1007/BF01318360
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01318360