Summary
A set of 41 overlapping peptides, representing the complete sequence of SFV-E2 protein were synthesized and analyzed in the ELISA test against murine anti-SFV sera. No single peptide was recognized by all antisera. Eight peptides were found to be highly reactive with hyperimmune anti-SFV sera. Six out of the eight peptide sequences coincide with the most hydrophilic regions of SFV-E2. Out of these, four peptides (amino acid positions 16–35, 61–80, 166–185, 286–305) that contain the least number of alphavirus conserved residues were selected. This panel constitutes the minimal number of peptides necessary and sufficient for specific recognition of hyperimmune mouse anti-SFV sera.
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Ariel, N., Lehrer, S., Elhanaty, E. et al. Serologically defined linear epitopes in the E2 envelope glycoprotein of Semliki Forest virus. Archives of Virology 113, 99–106 (1990). https://doi.org/10.1007/BF01318358
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DOI: https://doi.org/10.1007/BF01318358