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A novel method of typing rhinoviruses using the product of a polymerase chain reaction

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Summary

At present rhinoviruses are detected and serotyped in tissue cultures, a slow and laborious process. Previously we have described how the polymerase chain reaction can be used as a rapid method for detecting the presence of a rhinovirus, or enterovirus, in clinical samples without the need to culture. Here we describe a new method which uses the product of the polymerase chain reaction to determine the type of the rhinovirus. The technique is rapid and simple and should eventually greatly facilitate studies on rhinovirus infections.

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References

  1. Al-Nakib W, Stanway G, Forsyth M, Hughes PJ, Almond JW, Tyrrell DAJ (1986) Detection of human rhinoviruses and their molecular relationships using cDNA probes. J Med Virol 20: 289–296

    Google Scholar 

  2. Bruce CB, Al-Nakib W, Almond JW, Tyrrell DAJ (1989) Use of synthetic oligonuceotide probes to detect rhinovirus RNA. Arch Virol 105: 179–187

    Google Scholar 

  3. Cooney MK, Fox JP, Kenney GE (1982) Antigenic groupings of 90 rhinovirus serotypes. Infect Immun 37: 642–647

    Google Scholar 

  4. Forsyth M, Al-Nakib W, Chadwick P, Stanway G, Hughes PJ, Leckie G, Amond JW, Tyrrell DAJ (1989) Rhinovirus detection using probes from the 5′ and 3′ end of the genome. Arch Virol 107: 55–63

    Google Scholar 

  5. Gama RE, Horsnell P, Hughes PJ, North C, Bruce CB, Stanway G (1989) Amplification of rhinovirus specific nucleic acids from clinical samples using the polymerase chain reaction—a novel detection procedure. J Med Virol 28: 73–77

    Google Scholar 

  6. Gama RE, Hughes PJ, Bruce CB, Stanway G (1988) Polymerase chain reaction amplification of rhinovirus nucleic acids from clinical material. Nucleic Acids Res 16: 9346

    Google Scholar 

  7. Hamparian VV, Colonno RJ, Cooney MK, Dick EC, Gwaltney JM, Hughes JH, Jordan WS, Kapikian AZ, Mogabgab WJ, Monto A, Philips CA, Ruckert RR, Schieble JH, Stott EJ, Tyrrell DAJ (1987) A collaborative report: rhinoviruses—extension of the numbering system from 89 to 100. Virology 159: 191–192

    Google Scholar 

  8. Hyypiä T, Auvinen P, Maaronen M (1989) Polymerase chain reaction for human picornaviruses. J Gen Virol 70: 2943–2952

    Google Scholar 

  9. Kellner G, Popow-Kraupp T, Kundi M, Binder C, Wallner H, Kunz C (1988) Contribution of rhinoviruses to respiratory infections in childhood: a prospective study in a mainly hospitalized infant population. J Med Virol 25: 455–469

    Google Scholar 

  10. Stott EJ, Killington RA (1972) Rhinoviruses. Annu Rev Microbiol 26: 503–525

    Google Scholar 

  11. Torgersen H, Skern TM, Blaas D (1989) Typing of human rhinoviruses based on sequence variations in the 5′ non-coding region. J Gen Virol 70: 3111–3116

    Google Scholar 

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Bruce, C.B., Gama, R.E., Hughes, P.J. et al. A novel method of typing rhinoviruses using the product of a polymerase chain reaction. Archives of Virology 113, 83–87 (1990). https://doi.org/10.1007/BF01318355

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  • DOI: https://doi.org/10.1007/BF01318355

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