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Relationship between plasma and hepatic cytosolic levels of ornithine decarboxylase (ODC) and thymidine kinase (TK) in 70% hepatectomized rats

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Abstract

Ornithine decarboxylase (ODC) and thymidine kinase (TK) are enzymes important for DNA synthesis, a process that is critical for cell renewal and regeneration. As such, they already have been used as surrogate markers of regeneration in tissue. In the present study, the activity of these two enzymes in plasma of rats and regenerating hepatic tissue following a 70% hepatectomy were determined. The results demonstrate that the changes in these enzyme activities in plasma reflect the changes obtained in the liver tissue. Thus, blood levels of ODC and TK can be used as a less invasive and nondestructive means of monitoring the regenerative response of the liver and possibly other tissues.

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Authors and Affiliations

  1. Department of Gastroenterology, University of Bari, Italy

    Lorenzo Polimeno PhD, Alessandro Azzarone MD, Paola Dell'Aquila MD, Cinzia Amoruso MD, Michele Barone MD, Annamaria Angelini MD, David H. Van Thiel MD & Antonio Francavilla MD

  2. Department of Surgery, University Health Center of Pittsburgh, University of Pittsburgh, Pittsburgh, Pennsylvania

    Lorenzo Polimeno PhD, Alessandro Azzarone MD, Paola Dell'Aquila MD, Cinzia Amoruso MD, Michele Barone MD, Annamaria Angelini MD, David H. Van Thiel MD & Antonio Francavilla MD

  3. Veterans Administration Medical Center, Pittsburgh, Pennsylvania

    Lorenzo Polimeno PhD, Alessandro Azzarone MD, Paola Dell'Aquila MD, Cinzia Amoruso MD, Michele Barone MD, Annamaria Angelini MD, David H. Van Thiel MD & Antonio Francavilla MD

Authors
  1. Lorenzo Polimeno PhD
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  2. Alessandro Azzarone MD
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  3. Paola Dell'Aquila MD
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  4. Cinzia Amoruso MD
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  5. Michele Barone MD
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  6. Annamaria Angelini MD
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  7. David H. Van Thiel MD
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  8. Antonio Francavilla MD
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Additional information

Supported by research grants from the Veterans Administration and project grant AM29961 from the National Institutes of Health, Bethesda, Maryland; by grant 87/0129144 from the Consiglio Nazionale dell Richerche, Italy.

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Polimeno, L., Azzarone, A., Dell'Aquila, P. et al. Relationship between plasma and hepatic cytosolic levels of ornithine decarboxylase (ODC) and thymidine kinase (TK) in 70% hepatectomized rats. Digest Dis Sci 36, 289–292 (1991). https://doi.org/10.1007/BF01318198

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  • DOI: https://doi.org/10.1007/BF01318198

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