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The effect of arginine deprivation on the cytopathogenic effect and replication of human cytomegalovirus
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  • Published: June 1975

The effect of arginine deprivation on the cytopathogenic effect and replication of human cytomegalovirus

  • Helen M. Garnett1,2 

Archives of Virology volume 48, pages 131–145 (1975)Cite this article

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Summary

Arginine is necessary for the development of the cytopathogenic effect of human cytomegalovirus in human embryonic fibroblasts. It is also required, though in greater concentrations, for the production of infective virions, the requirement being at an early stage of replication. Inhibitor studies suggested that this block in replication caused by arginine deficiency was prior to the formation of viral DNA. Withdrawal of arginine from the medium 24 or 48 hours after infection resulted in a decline in virus production indicating that the continued presence of the amino acid is necessary for constant virus production. Infected cultures deprived of arginine could be stimulated to produce cytopathic effects and infective virions by replacement of the amino acid even eight days after inoculation, demonstrating that the information for cytomegalovirus replication remains intact within the cell. This establishment of latencyin vitro may be related to the ability of the virus to establish a similar statein vivo.

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Author information

Authors and Affiliations

  1. Poliomyelitis Research Foundation, Johannesburg, South Africa

    Helen M. Garnett

  2. Department of Botany and Microbiology, University of the Witwatersrand, Jan Smuts Avenue, Johannesburg, South Africa

    Helen M. Garnett

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  1. Helen M. Garnett
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Garnett, H.M. The effect of arginine deprivation on the cytopathogenic effect and replication of human cytomegalovirus. Archives of Virology 48, 131–145 (1975). https://doi.org/10.1007/BF01318146

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  • Received: 06 June 1974

  • Accepted: 09 January 1975

  • Issue Date: June 1975

  • DOI: https://doi.org/10.1007/BF01318146

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Keywords

  • Infectious Disease
  • Infective Virion
  • Arginine
  • Great Concentration
  • Cytopathic Effect
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