Summary
A total of 72 Borna Disease virus infected rabbits were treated with different concentrations of cyclophosphamide, glucocorticoids or both in combination. Comparison with untreated, infected rabbits showed a drastic alteration in the clinical picture, a considerable prolongation of the survival time, and differences in weight and body temperature during the course of the disease. The immunosuppressed animals had no or low amounts of antibodies in the serum and cerebrospinal fluid, but they harbored infectious virus and high amounts of specific antigen in the brain. A quotient of the relative amount of antibodies and antigen never exceeded 0.20 and was significantly lower than in untreated rabbits. Immunohistologically, differences in location and distribution of antigen as compared to positive untreated animals could not be detected. In the immunosuppressed animals perivascular infiltrates were not observed in the different regions of the brain.
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Axelsen, N. H., Krøll, J., Weeke, B.: A manual of quantitative immuno-electrophoresis; methods and applications. Oslo-Bergen-Tromsø: Universitetsforlaget 1975.
Buckley, C. E., Gills, J. P.: Cyclophosphamide therapy of peripheral uveitis. Arch. Intern. Med.124, 29–35 (1969).
Cole, G. A., Gilden, D. H., Monjan, A. A., Nathanson, N.: Lymphocytic choriomeningitis virus, pathogenesis of acute central nervous system disease. Fed. Proc.30, 1831–1841 (1971).
Fenner, F., McAuslan, B. R., Mims, C. A., Sambrook, J., White, D. O.: The biology of animal viruses. New York-London: Academic Press 1974.
Kobune, F., Chino, F., Yamanouchi, K.: Studies on recovery mechanism from rinderpest virus infection in rabbits. 1. Effect of antithymocyte serum and thymectomy. Japan J. med. Sci. Biol.29, 265–275 (1976).
Krey, H. F., Ludwig, H., Gierend, M.: Borna Disease virus induced retinouveitis treated with immunosuppressive drugs. Albrecht v. Graefe's Arch. klin. exp. ophthalmol. (1980, in press).
Krey, H. F., Ludwig, H., Boschek, C. B.: Multifocal retinopathy in Borna disease virus infected rabbits. Amer. J. Ophthalmol.87, 157–164 (1979).
Lennette, E. H.: General principles underlying laboratory diagnosis of viral and rickettsial infections. In: Diagnostic procedures for viral and rickettsial diseases, 4th ed. (Lennette, E. H., Schmidt, N. J., eds.), 1–65. New York: The American Public Health Association, Inc. 1969.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin-phenol reagent. J. biol. Chem.193, 255–275 (1951).
Ludwig, H., Becht, H.: Borna disease and its causative agent (Workshop, Würzburg 1975). In:Ter Meulen, V., Katz, M. (eds.), Slow Virus Infections of the Central Nervous System. Investigational Approaches to Etiology and Pathogenesis of These Diseases, 75–83. New York: Springer 1977.
Ludwig, H., Becht, H., Croh, L.: Borna disease (BD), a slow virus infection. Med. Microbiol. Immunol. (Berl.)158, 275–289 (1973).
Ludwig, H., Becht, H., Rott, R.: Neuere Erkenntnisse über die Borna'sche Krankheit. Tierärztl. Umschau32, 347–351 (1977).
Ludwig, H., Koester, V., Pauli, G., Rott, R.: The cerebrospinal fluid of rabbits infected with Borna disease virus. Arch. Virol.55, 209–223 (1977).
Ludwig, H., Thein, P.: Demonstration of specific antibodies in the central nervous system of horses naturally infected with Borna disease virus. Med. Microbiol. Immunol.163, 215–226 (1977).
Meulen, ter V., Hall, W. W.: Slow virus infections of the nervous system: Virological, immunological and pathogenetic considerations. J. gen. Virol.41, 1–25 (1978).
Nathanson, N., Panitch, H., Palsson, P. A., Petursson, G., Georgsson, G.: Pathogenesis of Visna II. Effect of immunosuppression upon early central nervous system lesions. Lab. Invest.35, 444–451 (1976).
Nicolau, S., Galloway, I. A.: Borna disease and enzootic encephalomyelitis of sheep and cattle. Privy Council Medical Research Council Special Report Series121, 7–90 (1928).
Nitzschke, E.: Untersuchungen über die experimentelle Bornavirus-Infektion bei der Ratte. Zbl. Vet. Med.B 10, 470–527 (1963).
Paterson, P. Y.: Neuroimmunologic diseases of animals and humans. Rev. inf. Dis.1, 468–482 (1979).
Porter, D. D.: Destruction of virus-infected cells by immunological mechanisms. Ann. Rev. Microbiol.25, 283–290 (1971).
Sprankel, H., Richarz, K., Ludwig, H., Rott, R.: Behavior alterations in tree shrews (Tupaia glis, Diard 1820) induced by Borna Disease virus. Med. Microbiol. Immunol.165, 1–18 (1978).
Wagner, K., Ludwig, H., Paulsen, J.: Fluoreszenz-serologischer Nachweis von Borna-Virus Antigen. Berl. Münch. Tierärztl. Wschr.81, 395–396 (1968).
Zwick, W.: Bornasche Krankheit und Encephalomyelitis der Tiere. In:Gildemeister, E., Haagen, E., Waldmann, O. (eds.): Handb. der Viruskrankheiten, 252–354, Jena: Fischer 1939.
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Gierend, M., Ludwig, H. Influence of immunosuppressive treatment on Borna Disease in rabbits. Archives of Virology 67, 217–228 (1981). https://doi.org/10.1007/BF01318132
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DOI: https://doi.org/10.1007/BF01318132