Summary
The association of human cytomegalovirus with mink and rabbit lung cells was studied. Strain AD-169 was used which was free of Mycoplasma and other contaminating agents. It was found to be incapable of productively infecting mink lung cells. Infection appeared to be initiated but aborted at an early stage. This was indicated by indirect immunofluorescence, assays of culture supernatants and cell lysates for infectious virus, electron microscopy of ultra-thin sections of infected cells, labelling of virus and viral DNA with3H-thymidine and assay of virally-induced DNA polymerase at various times after infection. On the other hand, using these methods, AD-169 was found to infect rabbit lung cells, the virus being produced in low amounts over a period of up to one month after infection. At this time, focal areas of infection were still apparent and 15 per cent of the cells expressed nuclear viral antigens as shown by immunofluorescence. The viral genome was assumed to have become latent in some rabbit cells with a few being capable of producing infectious virus.
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References
Boldogh, I., Gonczol, E., Gartner, L., Vaczi, L., Michelson, S.: Latent infection of mouse cells with human cytomegalovirus. Bull. Cancer (Paris)63, 411–419 (1976).
Darai, G., Flugel, R. M.: Mink lung cells: a non-primate cell line highly susceptible for varicella-zoster virus. J. gen. Virol.41, 179–181 (1978).
Farber, I., Wutzler, P., Schweizer, H., Sprossig, M.: Human cytomegalovirus induced changes in rabbit cells. Arch. Virol.59, 257–261 (1979).
Figueroa, M. E., Geder, L., Rapp, F.: Infection of human amnion cells with cytomegalovirus. J. med. Virol.2, 369–375 (1978).
Fioretti, A., Furukawa, T., Santioli, D., Plotkin, S.: Non-productive infection of guinea pig cells with human cytomegalovirus J. Virol.11, 998–1003 (1973).
Grayzel, A. I.: Uridine incorporation into the media and RNA of cultured rheumatoid synovial cells. Arthritis Rheum.16, 419–421 (1973).
Hamelin, C., Lussier, G.: Concentration of human cytomegalovirus from large columns of tissue culture fluids. J. gen. Virol.42, 193–197 (1979).
Hirai, K., Furukawa, T., Plotkin, S.: Induction of DNA polymerase in WI-38 and guinea pig cells infected with human cytomegalovirus. Virology70, 251–255 (1976).
Huang, E.-S.: Human cytomegalovirus. III. Virus-induced DNA polymerase. J. Virol.16, 298–310 (1975).
Huang, E.-S., Chen, S.-T., Pagano, J. S.: Human cytomegalovirus. I. Purification and characterization of viral DNA. J. Virol.12, 1473–1481 (1973).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1971).
Michelson-Fiske, S., Arnoult, J., Febvre, H.: Cytomegalovirus infection of human lung epithelial cellsin vitro. Intervirology2, 354–363 (1975).
Mocarski, E. S., Stinski, M. F.: Persistence of the cytomegalovirus genome in human cells. J. Virol.31, 761–775 (1979).
Norval, M., Marmion, B. P.: Attempts to identify viruses in rheumatoid synovial cells. Ann. Rheum. Dis.35, 106–113 (1976).
St Jeor, S., Rapp, R.: Cytomegalovirus: conversion of nonpermissive cells to a permissive state for virus replication. Science181, 1060–1061 (1973).
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Hart, H., Norval, M. Association of human cytomegalovirus (HCMV) with mink and rabbit lung cells. Archives of Virology 67, 203–215 (1981). https://doi.org/10.1007/BF01318131
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DOI: https://doi.org/10.1007/BF01318131