Abstract
Vinblastine treatment of superinduced human fibroblast (FS-4) cultures strongly inhibited interferon (IFN) secretion and resulted in intracellular accumulation of IFN activity. The cell-associated IFN migrated slower than secreted IFN in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, indicating a larger size. The results suggest the presence of a possible precursor form of mature Hu IFN-β with biological activity.
This is a preview of subscription content, log in via an institution to check access.
References
Blobel, G., Dobberstein, B.: Transfer of proteins across membranes: I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane bound ribosomes of murine myeloma. J. Cell. Biol.67, 835–851 (1975).
Blobel, G., Walter, P., Chang, C. N., Goldman, B., Erickson, A., Lingappa, V.: Translocation of proteins across membrane-signal hypothesis and beyond. Symp. Soc. Exp. Biol.33, 9–36 (1979).
Davis, B. D., Tai, P. C.: The mechanism of protein secretion across membranes. Nature283, 433–438 (1980).
Derynck, R., Content, J., DeClercq, E., Volckaert, G., Tavernier, J., Devos, R., Fiers, W.: Isolation and structure of a human fibroblast interferon gene. Nature285, 542–546 (1980).
Havell, E. A., Vilcek, J.: Inhibition of interferon secretion by vinblastine. J. Cell. Biol.64, 716–719 (1975).
Houghton, M., Eaton, M. A. W., Stewart, A. G., Smith, J. C., Doel, S. M., Catlin, G. H., Lewis, H. M., Patel, T. P., Emtage, J. S., Carey, N. H., Porter, A. G.: The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligo deoxyribonucleotide primers of reverse transcriptase. Nucl. Acid. Res.8, 2885–2894 (1980).
Knight, E., jr., Fahey, D.: Human fibroblast interferon. An improved purification. J. Biol. Chem.256, 3609–3611 (1981).
Lin, L. S., Wiranowska-Stewart, M., Chudzio, T., Stewart II, W. E.: Characterization of the heterogeneous molecules of human interferons: Differences in the cross species antiviral activities of various molecular populations in human leukocyte interferons. J. gen. Virol.39, 125–130 (1978).
Okamura, H., Berthold, W., Hood, L., Hunkapiller, M., Inoue, M., Smith-Johannsen, H., Tan, Y. H.: Human fibroblastoid interferon: Immunosorbent column chromatography and N-terminal amino acid sequence. Biochemistry19, 3831–3835 (1980).
Sehgal, P. B., Sagar, A.: Heterogeneity of poly (I) · poly (C)-induced human fibroblast interferon mRNA species. Nature288, 95–97 (1980).
Stein, S., Kenny, C., Frisen, H.-J., Shively, J., Valle, V. D., Pestka, S.: NH2-terminal amino acid sequence of human fibroblast interferon. Proc. Natl. Acad. Sci. U.S.A.77, 5716–5719 (1980).
Stewart II, W. E.: The Interferon System. Wien-New York: Springer 1979.
Stewart II, W. E., Sarkar, F. H., Taira, H., Hall, A., Nagata, S., Weissmann, C.: Comparison of several biological and physicochemical properties of human leukocyte interferons produced by human leukocytes and byE. coli. Gene11, 181–186 (1980).
Taniguchi, T., Fujii-Kuriyama, Y., Muramatsu, M.: Molecular cloning of human interferon cDNA. Proc. Natl. Acad. Sci. U.S.A.77, 4003–4006 (1980).
Weissenbach, J., Chernajovsky, Y., Zeevi, M., Shulman, L., Soreq, H., Nir, U., Wallach, D., Perricaudet, M., Tiollais, P., Revel, M.: Two interferon mRNAs in human fibroblasts:In vitro translation andEscherichia coli cloning studies. Proc. Natl. Acad. Sci. U.S.A.77, 7152–7156 (1980).
Wiranowska-Stewart, M., Chudzio, T., Stewart II, W. E.: Repeated super-induction of interferon in human diploid fibroblast cultures. J. gen. Virol.37, 221–223 (1980).
Author information
Authors and Affiliations
Additional information
With 3 Figures
Rights and permissions
About this article
Cite this article
Sarkar, F.H., Gupta, S.L. Inhibition of interferon secretion in human fibroblast by vinblastine. Archives of Virology 73, 343–349 (1982). https://doi.org/10.1007/BF01318088
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01318088