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Sensitivity of bovid herpesvirus 2 replication to temperatures found in the natural host

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Summary

Two isolates of bovid herpesvirus 2 replicated poorly in bovine testicular cells and fetal kidney cells at 39–40° C, temperatures commonly observed in virus-infected cattle. High viral titers occurred in replicate cultures at 30–37° C. Persistent viral infections were noted in cultures maintained at 40° C. Interferon-like activity was not responsible for the high-temperature restriction since the level was not significantly different between virus-infected cultures incubated at 35° or 40° C. Spontaneous viral inactivation was only three times as rapid at 41° as at 35° C. Analysis of temperature shift experiments with respect to results of growth studies and electron microscopy indicated a temperature sensitive event late in replication. The sensitiv of bovid herpesvirus 2 replication to elevated temperature provides a possible explanation for the extensive viral growth and occasionally severe lesions which are limited exclusively to the skin of infected cattle.

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Authors and Affiliations

  1. James A. Baker Institute for Animal Health, Cornell University, Ithaca, New York, USA

    G. J. Letchworth III, L. E. Carmichael & Helen A. Greisen

  2. USDA, ARS, Plum Island Animal Disease Center, P.O. Box 848, 11944, Greenport, NY, USA

    G. J. Letchworth III

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  1. G. J. Letchworth III
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  2. L. E. Carmichael
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  3. Helen A. Greisen
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Letchworth, G.J., Carmichael, L.E. & Greisen, H.A. Sensitivity of bovid herpesvirus 2 replication to temperatures found in the natural host. Archives of Virology 73, 273–286 (1982). https://doi.org/10.1007/BF01318081

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  • DOI: https://doi.org/10.1007/BF01318081

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