Summary
A method is described for the serotyping and subgrouping of rotaviruses by enzyme-linked immuno-sorbent assay (ELISA). For the isolates tested “blind” under code in parallel the serotyping results obtained by ELISA and serum neutralization of fluorescent focus formation were the same. After absorption of the typing antisera with purified heterotypic rotavirus, up to 128-fold differences in titres between isolates were observed in the ELISA test. The results of serotyping and subgrouping, by ELISA, of strains previously described and characterized by the authors' and other laboratories are also given. An attempt is made to correlate the serological findings with variations between virus strains based upon patterns of genome molecular weights as revealed by polyacrylamide gel electrophoresis. The application of this ELISA technique to epidemiological studies and vaccine research is discussed.
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Beards, G. M., Pilfold, J. N., Thouless, M. E., Flewett, T. H.: Rotavirus serotypes by serum neutralisation. J. Med. Virol.5, 231–237 (1980).
Beards, G. M.: A method for the purification of rotaviruses and adenoviruses from faeces. J. Virol. Meth. In press 1982.
Bridger, J. C.: Location of type specific antigens in calf rotavirus. J. Clin. Microbiol.8, 625–628 (1978).
Bryden, A. S., Davies, H. A., Thouless, M. E., Flewett, T. H.: Diagnosis of rotavirus infection by cell culture. J. Med. Microbiol.10, 121–125 (1975).
Drosdov, S. G., Shekoyan, L. A., Korolev, M. B., Andzhaparidze, A. G.: Human rotavirus in cell culture: isolation and passaging. Voprosy Virusologii4, 389–392 (1979). (In Russian.)
Espejo, R. T., Munoz, O., Serafin, F., Romero, P.: Shift in the prevalent human rotavirus detected by ribonucleic acid segment differences. Inf. Immun.27, 351–354 (1980).
Flewett, T. H., Thouless, M. E., Pilfold, J. N., Bryden, A. S., Candeias, J. A. N.: More serotypes of human rotavirus. Lancetii, 632 (1978).
Flewett, T. H., Woode, G. N.: The rotaviruses. Brief review. Arch. Virol.57, 1–23 (1978).
Greenberg, H. B., Kalica, A. R., Wyatt, R. G., Jones, R. W., Kapikian, A. Z., Chanock, R. M.: Rescue of non-cultivable human rotavirus by gene reassortment during mixed infection withts mutants of a cultivable bovine rotavirus. Proc. Natl. Acad. Sci.78, 420–424 (1981).
Kalica, A. R., Sereno, M. M., Wyatt, R. G., Mebus, C. A., Chanock, R. M., Kapikian, A. Z.: Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology87, 247–255 (1979).
Kalica, A. R., Greenberg, H. B., Espejo, R. T., Flores, J., Wyatt, R. G., Kapikian, A. Z., Chanock, R. M.: Distinctive RNA patterns of human rotavirus subgroups 1 and 2. Infect. Immun.33, 958–961 (1981).
Kapikian, A. Z., Cline, W. L., Greenberg, H. B., Wyatt, R. G., Kalica, A. R., Banks, C. E., James, H. D., Flores, J., Chanock, R. M.: Antigenic characterization of human and animal rotavirus by immune adherence haemagglutination assay (IAHA). Inf. Immun.33, 415–425 (1981).
Rodger, S. M., Bishop, R. F., Birch, C., McLean, B., Holmes, L. H.: Molecular epidemiology of human rotavirus in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. J. Clin. Microbiol.13, 272–278 (1981).
Sato, K., Inaba, Y., Shinozuka, T., Matsumoto, M.: Isolation of human rotavirus in cell cultures. Arch. Virol.69, 155–160 (1981).
Schnagl, R. D., Holmes, I. H.: Characteristics of the genome of human infantile enteritis virus (rotavirus). J. Virol.19, 267–270 (1976).
Thouless, M. E., Bryden, A. S., Flewett, T. H., Woode, G. N., Bridger, J. C., Snodgrass, D. R., Herring, J. A.: Serological relationships between rotaviruses from different species as studies by complement fixation and neutralisation. Arch. Virol.53, 287–289 (1977).
Thouless, M. E., Bryden, A. S., Flewett, T. H.: Serotypes of human rotavirus. Lanceti, 39 (1978).
Urasawa, T., Urasawa, S., Taniguchi, K.: Sequential passages of human rotavirus in MA-104 cells. Microbiol. Immunol.25, 1025–1035 (1981).
Woode, G. N., Bridger, J. C., Jones, J. M., Flewett, T. H., Bryden, A. S., Davies, H. A., White, G. B. B.: Morphological and antigenic relationships between viruses (rotaviruses) from acute gastroenteritis of children, calves, piglets, mice and foals. Infect. Immun.14, 804–810 (1976).
Zissis, G., Lambert, J. P.: Different serotypes of human rotaviruses. Lanceti, 39 (1978).
Zissis, G., Lambert, J. P.: Enzyme-linked immunosorbent assays for serotyping of human rotavirus strains. J. Clin. Microbiol.11, 1–5 (1982).
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Thouless, M.E., Beards, G.M. & Flewett, T.H. Serotyping and subgrouping of rotavirus strains by the ELISA test. Archives of Virology 73, 219–230 (1982). https://doi.org/10.1007/BF01318076
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DOI: https://doi.org/10.1007/BF01318076