Summary
The replication of virulent and attenuated strains of African swine fever virus (ASFV) was studied in pure cultures of swine macrophages. To ensure complete destruction of the macrophage monolayers about 50–100 times more virulent ASFV was needed than attenuated virus although both isolates could be used to establish persistently infected cultures.
Interferon did not appear to influence virus yields from such cultures.
Fluorescent and electron microscopy studies of infected macrophages suggested that the cycle of infection of the two isolates was different.
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References
Allison, A. C.: The Scientific Basis of Medicine Annual Reviews.Gilliland andFrancis (eds.). London: Athlone Press 1972.
Allison, A. C.: The Immune Response to Infectious Diseases. Transplant. Rev.19, 3–55 (1974).
Enjuanes, L., Cubero, I., Viñuela, E.: Sensitivity of macrophages from different species to African swine fever virus. J. gen. Virol.34, 455–463 (1977).
Greig, A., Plowright, W.: The excretion of two virulent strains of African swine fever virus by domestic pigs. J. Hyg.68, 673–682 (1970).
Hess, W. R.: African swine fever virus. Virology Monographs9, 1–33 (1971).
Hess, W. R., Cox, B. F., Heuschele, W. P., Stone, S. S.: Propagation and modification of African swine fever virus in cell cultures. Amer. J. vet. Res.26, 141–146 (1965).
Malmquist, W. A., Hay, D.: Hemadsorption and cytopathic effect produced by African swine fever virus in swine bone marrow and buffy coat cultures. Amer. J. vet. Res.21, 104–108 (1960).
Moulton, J., Coggins, L.: Synthesis and cytopathogenesis of African swine fever virus in porcine cell cultures. Amer. J. vet. Res.29, 219–232 (1968).
Wardley, R. C., Wilkinson, P. J.: The growth of African swine fever virus in pig monocytes and macrophages. J. gen. Virol.38, 183–186 (1977).
Wilkinson, P. J., Wardley, R. C.: The replication of African swine fever virus in pig endothelial cells. Brit. vet. J.134, 280–282 (1978).
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Wardley, R.C., Hamilton, F. & Wilkinson, P.J. The replication of virulent and attenuated strains of African swine fever virus in porcine macrophages. Archives of Virology 61, 217–225 (1979). https://doi.org/10.1007/BF01318056
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DOI: https://doi.org/10.1007/BF01318056