Summary
HeLa cells are non-permissive for the neurotropic suckling mouse strain of measles virus (MMV), but are permissive for cell-adapted Edmonston strain of measles virus (Edm). Fluorescence and electron microscopy demonstrated no membrane fluorescence and no membrane associated viral components, as well as characteristic lack of nuclear antigen after MMV infection of HeLa cells. This appearance differs markedly from the membrane, cytoplasmic and nuclear fluorescence after Edm infection of HeLa cells. Fluorescence microscopy demonstrates fusion of the two dissimilar syncytia after mixed infection. This suggests Edm envelopment of MMV nucleocapsids may be the means of MMV rescue in this system.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anisimova, E., Mares, I., Kyncl, F.: Morphological changes in nuclei of BSC-1 cells infected with measles virus. Arch. ges. Virusforsch.30, 1–6 (1970).
Horta-Barbosa, L., Fuccillo, D. A., London, W. T., Jabbour, J. T., Zeman, W., Sever, L. J.: Isolation of measles virus from brain cell cultures of two patients with subacute sclerosing panencephalitis. Proc. Soc. exp. Biol.132, 272–277 (1969).
Imagawa, D. T., Adams, J. M.: Propagation of measles virus in suckling mice. Proc. Soc. exp. Biol.98, 567–569 (1958).
Nakai, M., Imagawa, D. T.: Electronmicroscopy of measles virus replication. J. Virol.3, 187–197 (1969).
Oyanagi, S., ter Meulen, V., Katz, M., Koprowski, H.: Comparison of subacute sclerosing panencephalitis and measles viruses: and electron microscope study. J. Virol.7, 176–187 (1971).
Payne, F. E., Baublis, J. V., Itabashi, H. H.: Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. New Engl. J. Med.281, 585–589 (1969).
ter Meulen, V., Katz, M., Käckell, Y.-M., Barbanti-Brodano, G., Koprowski, H., Lennette, E. H.: Subacute sclerosing panencephalitis:in vitro characterization of viruses isolated from brain cells in culture. J. inf. Dis.126, 11–17 (1972).
ter Meulen, V., Katz, M., Käckell, Y.-M.: Properties of SSPE virus, tissue culture and animal studies. Annals clin. Res.5, 293–297 (1973).
Watkins, J. F.: Fusion of cells for virus studies and production of cell hybrids: In: Methods in Virology, Vol. 5, 1–32. New York: Academic Press 1971.
Wear, D. J., Rapp, F.: Latent measles virus infection of the hamster central nervous system. J. Immunol.107, 1593–1598 (1971).
Weil, M. L., Imagawa, D. T.: Measles virus: Induced transfer of biological properties. Arch. ges. Virusforsch.39, 344–352 (1972).
Author information
Authors and Affiliations
Additional information
With 6 Figures
Rights and permissions
About this article
Cite this article
Weil, M.L., Imagawa, D.T. Measles virus: Study of induced transfer of biological properties. Archives of Virology 51, 217–225 (1976). https://doi.org/10.1007/BF01318025
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01318025