Summary
Salivary glands from Hartley guinea pigs were experimentally infected with guinea pig cytomegalovirus (GPCMV) and examined by light and electron microscopy at different time intervals. Characteristic intranuclear and intracytoplasmic viral inclusions were observed in duct cells of infected animals. Viral inclusion counts and infectivity titers in the salivary gland reached maximum levels by 3 to 4 weeks after infection; infectivity persisted, though at reduced levels, for at least 30 weeks. Electron microscopic examination of viral inclusions revealed several developmental events including nucleocapsid assembly, envelopment of nucleocapsids at the inner nuclear membrane and their enclosure by a thin vacuolar membrane. While contained within cytoplasmic vacuoles, enveloped virions acquired surface spikes. Cytoplasmic vacuoles containing virions subsequently coalesced and discharged mature virions at the cell surface into the lumen of the salivary gland duct. The data indicate that the ultrastructural development of GPCMV in the guinea pig salivary gland shows many similarities to that of human cytomegalovirus in humans. The salivary gland may provide a primary locus for virus shedding and horizontal transmission of cytomegalovirus.
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References
Brodsky, I., Rowe, W. P.: Chronic subclinical infection with mouse salivary gland virus. Proc. Soc. exp. Biol. Med.99, 654–655 (1958).
Choi, Y. C., Hsiung, G. D.: Cytomegalovirus infection in guinea pigs. II. Transplacental and horizontal transmission. J. inf. Dis.138, 197–202 (1978).
Cole, R., Kuttner, A. G.: A filterable virus present in the submaxillary glands of guinea pigs. J. exp. Med.44, 855–873 (1926).
Connors, W. S., Johnson, K. P.: Cytomegalovirus infection in weanling guinea pigs. J. inf. Dis.134, 442–449 (1976).
Craighead, J. E., Kanich, R. E., Almeida, J. D.: Nonviral microbodies with viral antigenicity produced in cytomegalovirus-infected cells. J. Virol.10, 766–775 (1972).
Donnellan, W. L., Chantra-Umporn, S., Kidd, J. M.: The cytomegalic inclusion cell. An electron microscopic study. Arch. Pathol.82, 336–348 (1966).
Duncan, J. R., Ramsey, F. K., Switzer, W. P.: Electron microscopy of cytomegalic inclusion disease of swine (inclusion body rhinitis). Am. J. vet. Res.26, 939–947 (1965).
Fiala, M., Honess, R. W., Heiner, D. C., Heine, J. W., Jr., Murnane, J., Wallace, R., Guze, L. B.: Cytomegalovirus protein. I. Polypeptides on virions and dense bodies. J. Virol.19, 243–254 (1976).
Fong, C. K. Y., Bia, F., Hsiung, G. D., Madore, P., Chang, P. W.: Ultrastructural development of guinea pig cytomegalovirus in cultured guinea pig embryo cells. J. gen. Virol.42, 127–140 (1979).
Haguenau, F., Michelson-Fiske, S.: Cytomegalovirus: Nucleocapsid assembly and core structure. Intervirology5, 293–299 (1975).
Henson, D., Strano, A. J.: Mouse Cytomegalovirus: Necrosis of infected and morphologically normal submaxillary gland acinar cells during termination of chronic infection. Amer. J. Pathol.68, 183–202 (1972).
Jackson, L.: An intracellular protozoan parasite of the ducts of the salivary glands of the guinea pig. J. inf. Dis.26, 347–350 (1920).
Kawanishi, H., Takeda, T., Matsumoto, M.: Human cytomegalovirus infection: Electron and light microscopic observations of the parotid glands of an autopsy case. Acta Pathol. Jpn.17, 171–189 (1967).
Luetzeler, J., Heine, U.: Nuclear accumulation of filamentous herpes simplex virus DNA late during the replicative cycle. Intervirology10, 289–299 (1978).
Luse, S. A., Smith, M. G.: Electron microscopy of salivary gland viruses. J. exp. Med.107, 623–632 (1958).
Lussier, G., Berthiaume, L., Payments, P.: Electron microscopy of murine cytomegalovirus: Development of the virusin vivo andin vitro. Arch. ges. Virusforsch.46, 269–280 (1974).
Martin, A. M., Jr., Kurtz, S. M.: Cytomegalic inclusion disease. An electron microscopic histochemical study of the virus at necropsy. Arch. Pathol.82, 27–34 (1966).
Middlekamp, J. N., Patrizi, G., Reed, C. A.: Light and electron microscopic studies of the guinea pig cytomegalovirus. J. Ultrastruct. Res.18, 85–101 (1967).
Rosenbusch, C. T., Lucas, A. M.: Studies on the pathogenicity and cytological reactions of the submaxillary gland virus of the guinea pigs. Amer. J. Pathol.15, 303–338 (1939).
Ruebner, B. H., Hirano, T., Slusser, R., Osborn, J., Medearis, D. N.: Cytomegalovirus infection. Viral ultrastructure with particular reference to the relationship of lysosomes to cytoplasmic inclusion. Amer. J. Pathol.48, 971–989 (1966).
Smith, M. G.: The salivary gland viruses of man and animals (Cytomegalic inclusion disease). Prog. med. Virol.2, 171–202 (1959).
Tenser, R. B., Hsiung, G. D.: Comparison of guinea pig cytomegalovirus and guinea pig herpes-like virus: Pathogenesis and persistence in experimentally infected animals. Infect. Immun.13, 934–940 (1976).
Valicek, L., Smid, B., Pleva, V., Mensik, J.: Porcine cytomegalic inclusion disease virus. Electron microscopic study of the nasal mucosa. Arch. ges. Virusforsch.32, 19–30 (1970).
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Fong, C.K.Y., Bia, F. & Hsiung, G.D. Ultrastructural development and persistence of guinea pig cytomegalovirus in duct cells of guinea pig submaxillary gland. Archives of Virology 64, 97–108 (1980). https://doi.org/10.1007/BF01318013
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DOI: https://doi.org/10.1007/BF01318013