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Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains

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Summary

Two subgroup-specific monoclonal antibodies (MAb) raised in mice against group A human rotavirus were shown to react by immunoblotting with the trimeric form of VP6 of the homologous subgroup and successfully applied to development of new single-sandwich ELISA systems for rapid subgrouping of human strains. All of the 344 strains tested could be subgrouped, but for two of them prior propagation in cell cultures was required.

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Authors and Affiliations

  1. Virus Laboratory, Institute of Infectius Diseases, University of Pavia, IRCCS Policlinico San Matteo, Pavia

    G. Gerna, Antonella Sarasini, Maria Torsellini, Angela di Matteo, F. Baldanti & M. Parea

  2. CNR Institute of Experimental Medicine, Consiglio Nazionale delle Ricerche, Rome, Italy

    M. Battaglia

Authors
  1. G. Gerna
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  2. Antonella Sarasini
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  3. Maria Torsellini
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  4. Angela di Matteo
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  5. F. Baldanti
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  6. M. Parea
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  7. M. Battaglia
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Gerna, G., Sarasini, A., Torsellini, M. et al. Characterization of rotavirus subgroup-specific monoclonal antibodies and use in single-sandwich ELISA systems for rapid subgrouping of human strains. Archives of Virology 107, 315–322 (1989). https://doi.org/10.1007/BF01317927

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  • DOI: https://doi.org/10.1007/BF01317927

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