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A strategy simplifying site-directed mutagenesis in the CD4-binding region of HIV gp 120

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Summary

By site-directed mutagenesis, two unique restriction sites, surrounding the CD4-binding DNA region of HIV gp 120, were created. The mutations do not cause amino acid substitutions but generate a DNA fragment, which is stable when cloned into M13 for further mutagenesis and is also easy to reclone.

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Authors and Affiliations

  1. Department of Clinical Virology, University of Göteborg, Sweden

    Anna Hemming & S. Olofsson

  2. Department of Biochemistry and Biophysics, Chalmers University of Technology, Göteborg, Sweden

    L. Lundberg

Authors
  1. Anna Hemming
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  2. L. Lundberg
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  3. S. Olofsson
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Hemming, A., Lundberg, L. & Olofsson, S. A strategy simplifying site-directed mutagenesis in the CD4-binding region of HIV gp 120. Archives of Virology 107, 301–305 (1989). https://doi.org/10.1007/BF01317925

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  • DOI: https://doi.org/10.1007/BF01317925

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