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Inhibition of SV40 DNA replication by Rous sarcoma virus LTR enhancer

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Summary

Simian virus 40 (SV40) late region recombinant constructs containing the Rous sarcoma virus (RSV)src gene along with RSV enhancer stimulated expression but completely abolished SV40 DNA replication. Constructs, in which the heterologous enhancer sequences were omitted, did replicate normally in African green monkey kidney cells and, in the presence of helper virus, gave rise to infectious progeny. Inhibition of SV40 DNA replication follows acis-acting mechanism and is most likely due to a conformational change of the SV40 chromatin structure.

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References

  1. Cereghini S, Yaniv M (1984) Assembly of transfected DNA into chromatin: structural changes in the origin-promoter-enhancer region upon replication. EMBO J 3: 1243–1253

    Google Scholar 

  2. Cullen BR, Raymond K, Ju G (1985) Functional analysis of the transcriptional control region located within the avian retroviral Long Terminal Repeat. Mol Cell Biol 5: 438–447

    Google Scholar 

  3. Cullen BR, Raymond K, Ju G (1985) Transcriptional activity of avian retroviral Long Terminal Repeats directly correlates with enhancer activity. J Virol 53: 515–521

    Google Scholar 

  4. Czernilofsky P, Levinson AD, Varmus HE, Bishop JM, Tischer E, Goodman H (1983) Nucleotide sequence of an avian sarcoma oncogene (src) and proposed amino acid sequence of the gene product. Nature 287: 198–203

    Google Scholar 

  5. Czernilofsky P, Levinson AD, Varmus HE, Bishop JM, Tischer E, Goodman H (1983) Corrections of the nucleotide sequence of the src gene of Rous sarcoma virus. Nature 301: 736–738

    Google Scholar 

  6. DeLorbe WM, Luciw PA, Goodman H, Varmus HE, Bishop JM (1980) Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. J Virol 36: 50–61

    Google Scholar 

  7. Gluzman Y, Shenk T (1983) Enhancers and eukaryotic gene expression. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  8. Gong SS, Subramanian K (1988) Functional anatomy of the Simian virus 40 late promoter. Virology 163: 481–493

    Google Scholar 

  9. Gruss P, Rosenthal N, König M, Ellis RW, Shih TY, Scolnick EM, Khoury G (1982) The expression of viral and cellular p21 ras genes using SV40 as a vector. In: Gluzman Y (ed) Eukaryotic viral vectors. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 13–18

    Google Scholar 

  10. Hartman JR, Nayak DP, Fareed GC (1982) Expression of influenza virus hemagglutinin using SV40 vectors. In: Gluzman Y (ed) Eukaryotic viral vectors. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 19–27

    Google Scholar 

  11. Herr W, Clarke J (1986) The SV40 enhancer is composed of multiple functional elements that can compensate for one another. Cell 45: 461–470

    Google Scholar 

  12. Khoury G, Gruss P (1983) Enhancer elements (Review). Cell 33: 313–314

    Google Scholar 

  13. Kumar R, Firak TA, Schroll CT, Subramanian KN (1986) Activation of gene expression is adversely affected at high multiplicities of linked Simian virus 40 enhancer. Proc Natl Acad Sci USA 83: 3199–3203

    Google Scholar 

  14. Kumar R, Yoon KP, Subramanian KN (1988) Replication from a proximal Simian virus 40 origin is severely inhibited by multiple reiterations of the 72-base-pair repeat enhancer sequence. Mol Cell Biol 8: 1509–1517

    Google Scholar 

  15. Laimins LA, Tsichlis P, Khoury G (1984) Multiple enhancer domains in the 3′ terminus of the Prague strain of Rous sarcoma virus. Nucleic Acids Res 12: 6427–6442

    Google Scholar 

  16. Luciw PA, Bishop JM, Varmus HE, Capecchi MR (1983) Location and function of retroviral and SV40 sequences that enhance biochemical transformation after microinjection of DNA. Cell 33: 705–716

    Google Scholar 

  17. Lusky M, Botchan M (1981) Inhibition of SV40 replication in simian cells by specific pBR 322 DNA sequences. Nature 293: 79–81

    Google Scholar 

  18. Ondek B, Gloss L, Herr W (1988) The SV40 enhancer contains two distinct levels of organization. Nature 333: 40–45

    Google Scholar 

  19. Rübsamen H, Ziemiecki A, Friis RR, Bauer H (1980) The expression of pp60src and its associated protein kinase activity in cells infected with different transformation-defective, temperature-sensitive mutants of Rous sarcoma virus. Virology 102: 453–458

    Google Scholar 

  20. Sealey L, Chalkley (1987) At least two nuclear proteins bind specifically to the Rous sarcoma virus Long Terminal Repeat enhancer. Mol Cell Biol 7: 787–798

    Google Scholar 

  21. Tegtmeyer P, Ozer HJ (1971) Temperature-sensitive mutants of Simian virus 40: infection of permissive cells. J Virol 8: 516–524

    Google Scholar 

  22. Tooze J (ed) (1982) DNA tumour viruses, 9th edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  23. Weber F, de Villiers J, Schaffner W (1984) An SV40 “enhancer trap” incorporates exogenous enhancers or generates enhancers from its own sequences. Cell 36: 983–992

    Google Scholar 

  24. Weber F, Schaffner W (1985) Enhancer activity correlates with the oncogenic potential of avian retroviruses. EMBO J 4: 949–956

    Google Scholar 

  25. Zenke M, Grundstrom T, Matthes H, Wintzerith M, Schatz C, Wildeman A, Chambon P (1986) Multiple sequence motifs are involved in SV40 enhancer function. EMBO J 5: 387–397

    Google Scholar 

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Author notes

  1. Dorothea Binninger

    Present address: Paul-Ehrlich-Institut, Bundesamt für Sera und Impfstoffe, Frankfurt/Main, Germany

Authors and Affiliations

  1. Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt/Main

    Dorothea Binninger & Helga Rübsamen-Waigmann

  2. Paul-Ehrlich-Institut, Bundesamt für Sera und Impfstoffe, Frankfurt/Main, Germany

    F. -J. Ferdinand

Authors
  1. Dorothea Binninger
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  2. F. -J. Ferdinand
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  3. Helga Rübsamen-Waigmann
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Binninger, D., Ferdinand, F.J. & Rübsamen-Waigmann, H. Inhibition of SV40 DNA replication by Rous sarcoma virus LTR enhancer. Archives of Virology 107, 291–299 (1989). https://doi.org/10.1007/BF01317924

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  • DOI: https://doi.org/10.1007/BF01317924

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