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Analysis of DNA synthesis in herpes simplex virus infected cells by dual parameter flow cytometry

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Summary

The DNA incorporation of a thymidine analogue bromodeoxyuridine (BrdUrd) in herpes simplex virus type 2 (HSV-2) infected cervical cancer cell lines (CaSki and C-33 A) was studied by dual parameter flow cytometry. HSV-2 infection resulted in an exponential increase in DNA synthesis in the CaSki cells. In the less permissive C-33 A cells the proportion of DNA-synthesizing cells cycled during HSV-2 infection. The inhibition of viral DNA synthesis by phosphonoformate (PFA) was able to inhibit the virus induced changes in the CaSki but not in the C-33 A cells. In the C-33 A cells a part of the virus induced cellular DNA synthesis represents repair replication of cellular DNA.

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Authors and Affiliations

  1. Department of Biomedical Sciences, University of Tampere, Finland

    Matti Lehtinen, Pirkko Kulomaa & Pauli Leinikki

  2. Department of Clinical Chemistry, University Central Hospital, Tampere

    Olli-Pekka Kallioniemi

  3. Department of Clinical Sciences, University of Tampere, Tampere

    Jorma Paavonen

  4. Finnish Academy, Helsinki, Finland

    Jorma Paavonen

Authors
  1. Matti Lehtinen
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  2. Pirkko Kulomaa
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  3. Olli-Pekka Kallioniemi
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  4. Jorma Paavonen
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  5. Pauli Leinikki
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Lehtinen, M., Kulomaa, P., Kallioniemi, OP. et al. Analysis of DNA synthesis in herpes simplex virus infected cells by dual parameter flow cytometry. Archives of Virology 107, 215–223 (1989). https://doi.org/10.1007/BF01317918

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  • DOI: https://doi.org/10.1007/BF01317918

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