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Duration of challenge immunity to coronavirus JHM in mice


The duration of challenge resistance in mice immunized with mouse hepatitis virus (MHV) strain JHM was examined as a model of immunity to corona-virus infection. Genetically susceptible BALB/cByJ mice were immunized by intranasal (i.n.) or per os (p.o.) inoculation with MHV-JHM or sterile tissue culture fluid (sham) then challenged i.n. with MHV-JHM or sterile tissue culture fluid 1, 6, or 12 months later. Four days after challenge, virus in nasal turbinates and liver was quantified, and prevalance of microscopic lesions in liver and gut-associated lymphoid tissue was tabulated as indices of challenge resistance. MHV-immunized and challenged groups were compared to sham-immunized and challenged groups. Mice immunized by i.n. inoculation were strongly resistant to challenge at 1, 6, and 12 months. Mice immunized by p.o. inoculation were resistant at 1 month, but became partially susceptible to reinfection at 6 and 12 months, based upon all indices. These data indicate that, depending upon route of immunization, mice can become susceptible to reinfection with the same coronavirus strain over time.


  1. 1.

    Barthold SW (1988) Olfactory neural pathway in mouse hepatitis virus nasoencephalitis. Acta Neuropathol 76: 502–506

    Google Scholar 

  2. 2.

    Barthold SW, Beck DS, Smith AL (1986) Mouse hepatitis virus nasoencephalopathy is dependent upon virus strain and host genotype. Arch Virol 91: 247–256

    Google Scholar 

  3. 3.

    Barthold SW, Smith AL (1987) Response of genetically susceptible and resistant mice to intranasal inoculation with mouse hepatitis virus JHM. Virus Res 7: 225–229

    Google Scholar 

  4. 4.

    Barthold S, Smith AL (1989) Virus strain specificity of challenge immunity to coronavirus. Arch Virol 104: 187–196

    Google Scholar 

  5. 5.

    Frith CH, Ward JM (1988) Color atlas of neoplastic and non-neoplastic lesions in aging mice. Elsevier, New York, pp 10–26

    Google Scholar 

  6. 6.

    Holmes KV (1986) Replication of coronaviruses. In: Fields BN, Knipe DM, Chanock RM, Melnick JL, Roizman B, Shope RE (eds) Fundamental virology. Raven Press, New York, pp 513–525

    Google Scholar 

  7. 7.

    Hummel KP, Richardson FL, Fekete E (1975) Anatomy. In: Green EL (ed) Biology of the laboratory mouse. Dover, New York, pp 247–307

    Google Scholar 

  8. 8.

    Smith AL (1983) An immunofluorescence test for detection of serum antibody to rodent coronaviruses. Lab Anim Sci 33: 157–160

    Google Scholar 

  9. 9.

    Smith AL, Winograd DF (1986) Two enzyme immunoassays for detection of antibody to rodent coronaviruses. J Virol Methods 14: 335–343

    Google Scholar 

  10. 10.

    Wege H, Siddell S, ter Meulen V (1982) The biology and pathogenesis of coronaviruses. Curr Top Microbiol Immunol 99: 165–200

    Google Scholar 

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Barthold, S.W., Smith, A.L. Duration of challenge immunity to coronavirus JHM in mice. Archives of Virology 107, 171–177 (1989).

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  • Hepatitis
  • Infectious Disease
  • Tissue Culture
  • Lymphoid Tissue
  • Hepatitis Virus