Summary
Bluetongue virus multiplied in cell lines derived fromAedes albopictus andAedes pseudoscutellaris cells. Virus reached a maximum titre in theAe. pseudoscutellaris cells three days post inoculation, and inAe. albopictus cells six days p. i. Virus growth was demonstrated in both cell lines at 27° C and 37° C. Significant titres of virus were still present in theAe. albopictus cells after five subcultures at 27° C over a period of six weeks. No cytopathic effect was observed in either cell line.
A third cell line derived from the mosquitoAe. aegypti did not support the growth of Bluetongue virus.
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Jennings, M., Boorman, J. The susceptibility of cell lines ofAedes aegypti (Linn.),Aedes albopictus (Skuse) andAedes pseudoscutellaris (Theobald) to infection with Bluetongue virus. Archives of Virology 59, 121–126 (1979). https://doi.org/10.1007/BF01317901
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DOI: https://doi.org/10.1007/BF01317901