Summary
A vaccinia virus-specific cytotoxic protein (3−10×104 daltons) had previously been detected in extracts of infected HeLa cells. In this study the protein has been shown to be a late gene product and a virion component — almost certainly the monomer of the surface tubules of the vaccinia virion. The relationship of this cytotoxin to a number of early vaccinia-induced cytopathic effects was examined: it was not the mediator of vaccinia-induced early cell rounding, did not inhibit protein or RNA synthesis in cell-free systems or intact cells (after uptake-induction by hypertonic MgSO4), or cause the release of β-glucuronidase from a crude preparation of HeLa cell lysosomes. Its possible role in vaccinia-induced cell degeneration, late in infection, is discussed.
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Burgoyne, R.D., Stephen, J. Further studies on a vaccinia virus cytotoxin present in infected cell extracts: Identification as surface tubule monomer and possible mode of action. Archives of Virology 59, 107–119 (1979). https://doi.org/10.1007/BF01317900
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DOI: https://doi.org/10.1007/BF01317900