Summary
The ability of IgG anti-Fc and anti-Fab to neutralize infectious herpes simplex virus-IgG (HSV-IgG) complexes was determined. When limiting amounts of antiglobulin were used, antibody directed against the Fab portion of human IgG was significantly more effective than anti-Fc antibodies in neutralizing the HSV-IgG complexes. The detection of viral bound antibody was enhanced by the incorporation of heterologous antiglobulin or complement in the antiglobulin neutralization test. Specifically, HSV-IgG which had been incubated with rabbit antihuman globulin was further neutralized by goat antirabbit IgG or guinea pig serum complement. This augmented neutralization test could prove useful in detecting small amounts of antibody bound to virus in infectious isolates from patients or experimental animals with viral diseases.
Similar content being viewed by others
References
Ashe, W. K., Notkins, A. L.: Neutralization of an infectious herpes simplex virus-antibody complex by antiglobulin. Proc. Nat. Acad. Sci.56, 447–451 (1966).
Ashe, W. K., Daniels, C. A., Scott, G. S., Notkins, A. L.: Interaction of rheumatoid factor with infectious herpes simplex virus-antibody complexes. Science172, 176–177 (1971).
Daniels, C. A., Mergenhagen, S. E., Notkins, A. L.: Measurement of antibody against human IgG by the neutralization of IgG sensitized herpes simplex virus. J. Immunol.104, 470–474 (1970).
Daniels, C. A.: Mechanisms of Viral Neutralization. In:Notkins, A. L. (ed.), Viral Immunology and Immunopathology, 79. New York: Academic Press 1975.
Daniels, C. A., LeGoff, S. G., Notkins, A. L.: Shedding of infectious virus-antibody complexes from vesicular lesions of patients with recurrent herpes labialis. Lancetii, 524–534 (1975).
Dulbecco, R., Vogt, M., Strickland, A. G. R.: A study of the basic aspects of neutralization of two animal viruses, western equine encephalitis virus and poliomyelitis virus. Virology2, 162–205 (1956).
Fahey, J. L., McCoy, P. F., Goulair, M.: Chromatography of serum proteins in normal and pathologic sera: The distribution of protein bound carbohydrate and cholesterol, siderolipin, thyroxin binding protein, B12 binding protein, alkaline and acid phosphatases, radioiodinated albumin and myeloma protein. J. clin. Invest.37, 272–284 (1958).
Franklin, E. C.: Structural units of 7S gamma globulin. J. clin. Invest.39, 1933–1944 (1960).
Gipson, T. G., Daniels, C. A., Notkins, A. L.: Interaction of rheumatoid factor with infectious vaccinia virus-antibody complexes. J. Immunol.112, 2087–2093 (1974).
Goetzl, E. J., Metzgar, H.: Affinity labelling of a mouse myeloma protein which binds nitrophenyl lipids. Kinetics of labelling and isolation of labelled peptides. Biochem.9, 1267–1278 (1970).
Hahon, W.: Neutralization of residual infectivity of Venezuelan equine encephalomyelitis virus by anti-gamma globulin. J. gen. Virol.6, 361–372 (1970).
Hampar, B., Notkins, A. L., Mage, M., Keehn, M. A.: Heterogeneity in the properties of 7S and 19S rabbit neutralizing antibodies of herpes simplex virus. J. Immunol.100, 586–593 (1968).
Hirsch, M. S., Allison, A. C., Harvey, J. J.: Immune complexes in mice infected neonatally with Moloney leukaemogenic and murine sarcoma viruses. Nature223, 739–740 (1969).
Keller, R., Dwyer, J. E.: Neutralization of poliovirus by IgA coproantibodies. J. Immunol.101, 192–198 (1968).
Majer, M.: Virus sensitization. Curr. Top. Microbiol. Immunol.58, 69–84 (1972).
Notkins, A. L., Mahar, S., Scheele, C., Goffman, J.: Infectious virus antibody complexes in the blood of chronically infected mice. J. exp. Med.124, 81–97 (1966).
Oldstone, M. B. A., Dixon, F. J.: Immune complex disease in chronic viral infection. J. exp. Med.134, 32s–40s (1971).
Porter, D. D., Larsen, A. E., Porter, H. G.: The pathogenesis of Aleutian disease of mink. I.In vivo viral replication and the host antibody response to viral antigen. J. exp. Med.130, 575–593 (1969).
Singer, J. M., Altmann, G., Goldenberg, A., Platz, C. M.: The mechanism of particulate carrier reactions with rheumatoid sera. II. Sensitizing capacity of various human gamma globulins for latex particles. Arth. Rheum.3, 515–521 (1960).
Zalan, E., Bowman, E., Labzoffsky, N. A.: Infectious influenza virus-antibody complex. Arch. ges. Virusforsch.34, 209–213 (1971).
Author information
Authors and Affiliations
Additional information
With 4 Figures
Rights and permissions
About this article
Cite this article
Shivers, J.C., Daniels, C.A. Enhanced antiglobulin-mediated neutralization of herpes simplex virus-IgG complexes by complement and heterologous anti-immunoglobulin. Archives of Virology 59, 89–97 (1979). https://doi.org/10.1007/BF01317898
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01317898