Summary
Primary chick kidney cells were infected with avian infectious bronchitis virus (IBV) and examined by electron microscopy. Virus particles entered the cells by viropexis and distinction could be made between engulfment by cell processes (phagocytosis) and entry by micropinocytosis in coated transport vesicles.
Virus maturation occurred by budding into either the cisternae of the endoplasmic reticulum or cytoplasmic vacuoles, and evidence was obtained to suggest that the viral surface projections could be attached during the budding process. Late in infection large numbers of virus particles were present, mainly in cytoplasmic vacuoles, and the majority were released by cell lysis. Release by fusion of vacuoles with the plasma membrane was also observed, and individual virions could be transported from the endoplasmic reticulum to the surface within coated vesicles.
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Alexander, D. J., Collins, M.S.: Effect of pH on the growth and cytopathogenicity of avian infectious bronchitis virus in chick kidney cells. Arch. Virol.49, 339 to 348 (1975).
Apostolov, K., Flewett, T. H., Kendal, A. P.: In:Barry, R. D., Mahy, B. W. J. (eds.), The biology of large RNA viruses, 3–26. London-New York: Academic Press 1970.
Becker, W. B., McIntosh, K., Dees, J. H., Chanock, R. M.: Morphogensis of avian infectious bronchitis virus and a related human virus (strain 229E). J. Virol.1, 1019–1027 (1967).
Bracewell, C. D.: Antigenic relationships between strains of infectious bronchitis virus as shown by the plaque reduction test in chicken kidney cells. Proceedings Vth World Congress of the World Veterinary Poultry Association, Munich 1973 (1975).
Chardonnet, Y., Dales, S.: Early events in the interaction of adenoviruses with HeLa cells. I. Penetration of type 5 and intracellular release of the DNA genome. Virology40, 462–477 (1970).
Cunningham, C. H., Spring, M. P., Nazerian, K.: Replication of avian infectious bronchitis virus in African green monkey kidney cell line VERO. J. gen. Virol.16, 423–427 (1972).
David-Ferreira, J. F., Manaker, R. A.: An electron microscope study of the development of a mouse hepatitis virus in tissue culture cells. J. Cell Biol.24, 57–78 (1965).
Hamre, D., Kindig, D. A., Mann, J.: Growth and intracellular development of a new respiratory virus. J. Virol.1, 810–816 (1967).
Kapikian, A. Z., James, H. D., Kelly, S. J., Dees, J. H., Turner, H. C., McIntosh, K., Kim, H. W., Parrott, R. H., Vincent, M. M., Chanock, R. M.: Isolation from man of “avian infectious bronchitis virus-like” viruses (coronaviruses) similar to 229 E virus, with some epidemiological observations. J. inf. Dis.119, 282–290 (1969).
Nazerian, K., Cunningham, C. H.: Morphogenesis of avian infectious bronchitis in chicken embryo fibroblasts. J. gen. Virol.3, 469–470 (1968).
Oshiro, L. S.: Coronaviruses. In:Dalton, A. J., Haguenau, F. (eds.), Ultrastructure of animal viruses and bacteriophages: An atlas. New York: Academic Press 1973.
Oshiro, L. S., Schieble, J. H., Lennette, E. H.: Electron microscopic studies of coronavirus. J. gen. Virol.12, 161–168 (1971).
Roth, T. F., Porter, K. R.: Specialized sites on the cell surface for protein uptake. In:Breese, S. S. (ed.), Proc. 5th Int. Congr. Electron Microscopy, Philadelphia,2. Academic Press 1962.
Simpson, R. W., Hauser, R. E., Dales, S.: Viropexis of vesicular stomatitis virus by L cells. Virology37, 285–290 (1969).
Uppal, P. K., Chu, H. P.: An electron-microscope study of the trachea of the fowl infected with avian infectious bronchitis virus. J. med. Microbiol.3, 643–647 (1970).
Venable, J. H., Coggeshall, R.: A simplified lead citrate stain for use in electron microscopy. J. Cell Biol.25, 407–408 (1965).
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Chasey, D., Alexander, D.J. Morphogenesis of avian infectious bronchitis virus in primary chick kidney cells. Archives of Virology 52, 101–111 (1976). https://doi.org/10.1007/BF01317869
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DOI: https://doi.org/10.1007/BF01317869